David W. Holden
Imperial College London
32 Papers
55 Citations
David W. Holden is an academic researcher from Imperial College London. The author has contributed to research in topics: Salmonella enterica & Effector. The author has an hindex of 27, co-authored 32 publications.
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Papers
A pneumococcal pilus influences virulence and host inflammatory responses
Michèle A. Barocchi,J. Ries,X Zogaj,C Hemsley,Barbara Albiger,A Kanth,Sofia Dahlberg,Jenny Fernebro,Monica Moschioni,Vega Masignani,Kjell Hultenby,Annarita Taddei,Katharina Beiter,Florian Wartha,A von Euler,Antonello Covacci,David W. Holden,Staffan Normark,Rino Rappuoli,Birgitta Henriques-Normark +19 more
TL;DR: Evidence that initial bacterial adhesion and subsequent ability to cause invasive disease is enhanced by pili, long organelles able to extend beyond the polysaccharide capsule, previously unknown to exist in pneumococci is provided.
532
Functions of the Salmonella pathogenicity island 2 (SPI-2) type III secretion system effectors
Rita Figueira,David W. Holden +1 more
TL;DR: This review discusses the many different functions of effectors of Salmonella, which range from maintaining the integrity of the SCV and its juxtanuclear location, to interference with the host cytoskeleton and immune signalling.
368
Dynamics of intracellular bacterial replication at the single cell level
TL;DR: This work reports the development of a reporter system based on fluorescence dilution that enables direct quantification of the replication dynamics of Salmonella enterica serovar Typhimurium in murine macrophages at both the population and single-cell level and finds that, upon entry into macrophage, many bacteria do not replicate, but appear to enter a dormant-like state.
Salmonella Inhibits Retrograde Trafficking of Mannose-6-Phosphate Receptors and Lysosome Function
Kieran McGourty,Teresa L. M. Thurston,Sophie A. Matthews,Laurie Pinaud,Luís Jaime Mota,David W. Holden +5 more
TL;DR: It is suggested that Salmonella vacuoles undergo fusion with lysosomes whose potency has been reduced by SifA, and this work found that the effector S ifA subverted Rab9-dependent retrograde trafficking of MPRs, thereby attenuating lysOSome function.
pH Sensing by Intracellular Salmonella Induces Effector Translocation
TL;DR: neutral pH is identified as a physiological signal for effector translocation by intracellular Salmonella, which exploits the low pH of the vacuole as a signal to induce assembly of the secretion system, and then the neutral pH ofThe cytoplasm to trigger effectors translocation.