David M. Markusic
Indiana University
8 Papers
5 Citations
David M. Markusic is an academic researcher from Indiana University. The author has contributed to research in topics: Genetic enhancement & Vector (molecular biology). The author has an hindex of 5, co-authored 8 publications.
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Papers
Type I IFN Sensing by cDCs and CD4+ T Cell Help Are Both Requisite for Cross-Priming of AAV Capsid-Specific CD8+ T Cells.
Jamie L. Shirley,Geoffrey D. Keeler,Alexandra Sherman,Irene Zolotukhin,David M. Markusic,Brad E. Hoffman,Laurence Morel,Mark A. Wallet,Cox Terhorst,Roland W. Herzog,Roland W. Herzog +10 more
TL;DR: Mechanistic insights in the mechanisms that promote licensing of cDCs are sought, which is requisite for CD8+ T cell activation and inform the development of targeted immune interventions.
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B Cell Depletion Eliminates FVIII Memory B Cells and Enhances AAV8-coF8 Immune Tolerance Induction When Combined With Rapamycin
TL;DR: The data show that B and T cell immune modulation complements AAV8-coF8 gene therapy in naïve and inhibitor positive hemophilia A mice and suggest that such protocols should be considered for AAV gene Therapy in high risk or inhibitor positivehemophilia patients.
Development of a Clinical Candidate AAV3 Vector for Gene Therapy of Hemophilia B.
Harrison C. Brown,Christopher B. Doering,Roland W. Herzog,Chen Ling,David M. Markusic,H. Trent Spencer,Alok Srivastava,Arun Srivastava +7 more
TL;DR: An AAV3 vector incorporating a compact yet powerful liver-directed promoter as well as optimized hFIX cDNA sequence inserted between two AAV2 inverted terminal repeats (ITRs) is developed, predicted to achieve clinical efficacy at reduced vector doses for clinical gene therapy of hemophilia B.
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Adeno-Associated Virus D-Sequence-Mediated Suppression of Expression of a Human Major Histocompatibility Class II Gene: Implications in the Development of Adeno-Associated Virus Vectors for Modulating Humoral Immune Response.
Hyung-Joo Kwon,Keyun Qing,Selvarangan Ponnazhagan,Xu-Shan Wang,David M. Markusic,Siddhant Gupte,Shannon E. Boye,Arun Srivastava +7 more
TL;DR: The studies suggest that the D-sequence-mediated down-regulation of the MHC-II gene expression may be exploited towards the development of novel AAV vectors capable of dampening the host humoral response, which has important implication in the optimal use of these vectors in human gene therapy.
5
Preferential gene transfer of lentiviral vectors to liver-derived cells, using a hepatitis B peptide displayed on GP64.
TL;DR: Data suggest that the PreS1 peptide is involved in viral attachment to the cell surface and represents a potential approach for the targeting of lentiviral vectors to specific cell types.