David Almendral
Spanish National Research Council
13 Papers
2 Citations
David Almendral is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Peroxidase & Chemistry. The author has an hindex of 5, co-authored 8 publications.
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Papers
Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines.
Cristina Coscolín,Nadine Katzke,Antonio García-Moyano,José Navarro-Fernández,David Almendral,Mónica Martínez-Martínez,Alexander Bollinger,Rafael Bargiela,Christoph Gertler,Tatyana N. Chernikova,David Rojo,Coral Barbas,Hai Tran,Olga V. Golyshina,Rainhard Koch,Michail M. Yakimov,Gro Elin Kjæreng Bjerga,Peter N. Golyshin,Karl-Erich Jaeger,Manuel Ferrer +19 more
TL;DR: By an extensive analysis of the substrate spectra of 10 class III ω-TAs, a number of residues playing a role in determining the access and positioning of bulky ketones, bulky amines, and (R)- and (S) amines are identified, as well as of environmentally relevant polyamines, particularly putrescine.
Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores
Ana Robles-Martín,Rafael Amigot-Sánchez,Laura Fernandez-Lopez,Jose L. Gonzalez-Alfonso,Sergi Roda,Víctor Alcolea-Rodriguez,Diego Heras-Márquez,David Almendral,Cristina Coscolín,Francisco J. Plou,Raquel Portela,Miguel A. Bañares,Álvaro Martínez-del-Pozo,Sara García-Linares,Manuel Ferrer,Victor Guallar +15 more
TL;DR: Researchers engineered a protein nanopore, FraC, to deconstruct sub-micro- and nano-sized polyethylene terephthalate (nPET) through endo- and exo-type chain scission, producing various oligomers and terephthalic acid, with potential applications in nanotechnology and wastewater treatment.
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Enhancing the Hydrolytic Activity of a Lipase towards Larger Triglycerides through Lid Domain Engineering
Laura Fernandez-Lopez,Sergi Roda,Ana Robles-Martín,Rubén Muñoz-Tafalla,David Almendral,Manuel Ferrer,Víctor Guallar +6 more
TL;DR: The improvement of the lipase activity of a lipase retrieved from the Marine Metagenomics MarRef Database and assigned to the Actinoalloteichus genus is improved and redesigned mutants gain activity against bulkier triglycerides, such as glyceryl tridecanoate and tridodecanoate.
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Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes
Ramona Marasco,Marco Fusi,Cristina Coscolín,Alan Barozzi,David Almendral,Rafael Bargiela,Christina Nutschel,Christopher Pfleger,Jonas Dittrich,Holger Gohlke,Ruth Matesanz,Sergio Sanchez-Carrillo,Francesca Mapelli,Tatyana N. Chernikova,Peter N. Golyshin,Manuel Ferrer,Daniele Daffonchio +16 more
TL;DR: In this article , the authors quantified the thermal behavior of seven functionally independent enzyme classes (esterase, extradiol dioxygenase, phosphatase, beta-galactosidase, nuclease, transaminase, and aldo-keto reductase) in native proteomes of marine sediment microbiomes from the Irish Sea to the southern Red Sea.
Two-step functional screen on multiple proteinaceous substrates reveals temperature-robust proteases with a broad-substrate range.
Antonio García-Moyano,Yuleima Diaz,José Navarro,David Almendral,Pål Puntervoll,Manuel Ferrer,Gro Elin Kjæreng Bjerga +6 more
TL;DR: In this paper, a metagenome-derived protease was found to readily hydrolyze highly insoluble zein at temperatures up to 50 °C and pH 9 − 11.
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