Daniela Deharde
Charité
4 Papers
Daniela Deharde is an academic researcher from Charité. The author has contributed to research in topics: Biology & Gene. The author has an hindex of 4, co-authored 4 publications.
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Papers
Resolving the Combinatorial Complexity of Smad Protein Complex Formation and Its Link to Gene Expression.
Philippe Lucarelli,Marcel Schilling,Clemens Kreutz,Artyom Vlasov,Martin E. Boehm,Nao Iwamoto,Bernhard Steiert,Susen Lattermann,Marvin Wäsch,Markus Stepath,Matthias S. Matter,Mathias Heikenwalder,Katrin Hoffmann,Daniela Deharde,Georg Damm,Georg Damm,Daniel Seehofer,Daniel Seehofer,Maria Muciek,Norbert Gretz,Wolf D. Lehmann,Jens Timmer,Ursula Klingmüller +22 more
TL;DR: This work predicts and provides experimental evidence for the three most relevant Smad complexes in the mouse hepatoma cell line Hepa1-6 and specifies the contribution of each Smad complex to the expression of representative Smad target genes, and shows that these contributions are conserved in human hepatomacells and primary hepatocytes.
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Bile canaliculi formation and biliary transport in 3D sandwich-cultured hepatocytes in dependence of the extracellular matrix composition
Daniela Deharde,Christin Schneider,Thomas Hiller,Nicolas Fischer,Victoria Kegel,Marc Lübberstedt,Nora Freyer,Jan G. Hengstler,Tommy B. Andersson,Tommy B. Andersson,Daniel Seehofer,Johann Pratschke,Katrin Zeilinger,Georg Damm +13 more
TL;DR: The results of this study show that the choice of ECM has an impact on the morphology, cell assembly and bile canaliculi formation in PHH sandwich cultures.
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Context-specific flow through the MEK/ERK module produces cell- and ligand-specific patterns of ERK single and double phosphorylation.
Nao Iwamoto,Lorenza A. D'Alessandro,Sofia Depner,Bettina Hahn,Bernhard A. Kramer,Philippe Lucarelli,Artyom Vlasov,Markus Stepath,Martin Böhm,Daniela Deharde,Georg Damm,Daniel Seehofer,Wolf D. Lehmann,Ursula Klingmüller,Marcel Schilling +14 more
TL;DR: Insight is provided into how a single pathway can produce distinct responses, such as differentiation or proliferation, as well as the mitogen-activated protein kinase (MAPK) pathway, which can produce different cellular responses, depending on stimulus or cell type.
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Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells.
TL;DR: A more detailed protocol for the liver cell isolation process is provided including a video, which will allow an easy reproduction of this technique, which allows the isolation of PHH and NPC in high quality and quantity from one donor tissue sample.