Daniel Cole
University of Oxford
14 Papers
7 Citations
Daniel Cole is an academic researcher from University of Oxford. The author has contributed to research in topics: Microscopy & Fluorescence correlation spectroscopy. The author has an hindex of 4, co-authored 9 publications.
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Papers
Quantitative mass imaging of single biological macromolecules
Gavin Young,Nikolas Hundt,Daniel Cole,Adam Fineberg,Joanna Andrecka,Andrew Tyler,Anna Olerinyova,A Ansari,Erik G. Marklund,Miranda P. Collier,Shane A. Chandler,Olga Tkachenko,Joel D. Allen,Max Crispin,Neil Billington,Yasuharu Takagi,James R. Sellers,Cédric Eichmann,Philipp Selenko,Lukas Frey,Roland Riek,Roland Riek,Martin R. Galpin,Weston B. Struwe,Justin L. P. Benesch,Philipp Kukura +25 more
TL;DR: Interferometric scattering mass spectrometry allows spatiotemporally resolved measurement of a broad range of biomolecular interactions, one molecule at a time, to characterize the molecular dynamics of processes as diverse as glycoprotein cross-linking, amyloidogenic protein aggregation, and actin polymerization.
Label-Free Single-Molecule Imaging with Numerical-Aperture-Shaped Interferometric Scattering Microscopy
TL;DR: In this article, a partially transmissive spatial mask placed near the back focal plane of a high numerical aperture microscope objective was shown to enhance the extinction contrast of a scatterer near an interface by approximately T 1/2, where T is the transmissivity of the mask.
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Direct observation of the molecular mechanism underlying protein polymerization
TL;DR: This work uses mass photometry to quantify and monitor the full range of actin oligomers during polymerization with single-molecule sensitivity, and finds that traditional nucleation-based models cannot account for the observed distributions of actIn oligomers.
Quantitative mass imaging of single molecules in solution
Gavin Young,Nikolas Hundt,Daniel Cole,Adam Fineberg,Joanna Andrecka,Andrew Tyler,Anna Olerinyova,A Ansari,Erik G. Marklund,Miranda P. Collier,Shane A. Chandler,Olga Tkachenko,Joel D. Allen,Max Crispin,Neil Billington,Yasuharu Takagi,James R. Sellers,Cédric Eichmann,Philipp Selenko,Lukas Frey,Roland Riek,Martin R. Galpin,Weston B. Struwe,Justin L. P. Benesch,Philipp Kukura +24 more
TL;DR: Interferometric scattering mass spectrometry (iSCAMS) provides spatially resolved access to the dynamics of biomolecular interactions ranging from those involving small molecules to mesoscopic assemblies, one molecule at a time.
Myosin-5 varies its steps along the irregular F-actin track
Adam Fineberg,Yasuharu Takagi,Kavitha Thirumurugan,Joanna Andrecka,Neil Billington,Gavin Young,Daniel Cole,Stan A. Burgess,Alistair Curd,John A. Hammer,James R. Sellers,Philipp Kukura,Peter J. Knight +12 more
TL;DR: In this paper , the authors used high-resolution interferometric scattering (iSCAT) microscopy to reveal that myosin takes strides spanning 22 to 34 actin subunits, despite walking straight along the helical actin filament.
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