Daniel Chain
Weizmann Institute of Science
7 Papers
84 Citations
Daniel Chain is an academic researcher from Weizmann Institute of Science. The author has contributed to research in topics: Vitronectin & Protein kinase A. The author has an hindex of 5, co-authored 7 publications.
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Papers
Endogenous cleavage of the Arg-379-Ala-380 bond in vitronectin results in a distinct conformational change which 'buries' Ser-378, its site of phosphorylation by protein kinase A.
TL;DR: This paper shows that phosphorylation of vitronectin by protein kinase A is stoichiometric (approx. 1 mol/mol), that it is targeted to one site (Ser-378) at the C-terminal edge of the heparin-binding domain, and that it distinguishes between the two physiologically occurring forms of vitonectin.
The phosphorylation of the two‐chain form of vitronectin by protein kinase A is heparin dependent
TL;DR: Heparin or heparan sulfate are shown here to modulate the conformation of clipped vitronectin at physiological pH, exposing Ser378 and allowing its stoichiometric phosphorylation by the kinase.
31
An enzymatic assay for vitronectin based on its selective phosphorylation by protein kinase A.
TL;DR: The catalytic subunit (C) of cAMP-dependent protein kinase selectively phosphorylates vitronectin, a plasma protein that promotes cell adhesion and platelet aggregation, inhibits the inactivation of thrombin by antithrombin III, and participates in complement function.
18
Kit for assaying the cellular integrity of blood platelets
TL;DR: The invention relates to pharmaceutical compositions for the treatment of pathological conditions associated with impaired platelet function in humans, which comprises administering to a person in need a physiologically active quantity of a PKA inhibitor adapted to decrease or prevent vitronectin phosphorylation.
5
Patent
Kit or reagent for assaying the cellular integrity of blood platelets and pharmaceutical compositions containing a pka inhibitor
Daniel Chain,Beatriz Grodzicki,Shmuel Shaltiel +2 more
- 22 Dec 1989
TL;DR: In this article, the authors provided a kit or reagent for assaying the cellular integrity (intactness) of blood platelets comprising means for determining the quantity of PKA released by a number of platelets into a certain volume of plasma.
5