Oxidative pretreatment of lignocellulosic biomass for enzymatic hydrolysis: progress and challenges.

Soil physicochemical properties and vegetation types are the main factors affecting soil microorganisms, but there are few studies on the effects of the disturbance following volcanic eruption. To make up for this lack of knowledge, we used Illumina Miseq high-throughput sequencing to study the characteristics of soil microorganisms on both shores of a volcanically disturbed lake. Soil microorganisms in the two sites were subjected to different degrees of volcanic disturbance and showed significant heterogeneity. Mild volcanic disturbance area had higher enrichment of prokaryotic community. Co-occurrence network analysis showed that a total of 12 keystone taxa (9 prokaryotes and 3 fungi) were identified, suggesting that soil prokaryote may play a more significant role than fungi in overall community structure and function. Compared with severe volcanic disturbance area, the soil microbial community in mild volcanic disturbance area had the higher modular network (0.327 vs 0.291). The competition was stronger (positive/negative link ratio, P/N: 1.422 vs 1.159). Random forest analysis showed that soil superoxide dismutase was the most significant variable associated with soil microbial community. Structural equation model (SEM) results showed that keystone had a directly positive effect on prokaryotic (λ = 0.867, P < 0.001) and fungal (λ = 0.990, P < 0.001) multifunctionality while had also a directly positive effect on fungal diversity (λ = 0.553, P < 0.001), suggesting that keystone taxa played a key role in maintaining ecosystem stability. These results were important for understanding the effects of different levels of volcanic disturbance on soil ecosystems. 

Soil microbial community composition and co-occurrence network responses to mild and severe disturbances in volcanic areas.

This study introduces a cost-effective, mild thermal abiotic humification method for producing highly humified artificial humic acids (AHAs) from lignin-rich biogas digestate. Derived from the anaerobic digestion of cattle manure, the digestate slurry undergoes an MnO2-KOH-urea-enhanced humification reaction. Key process variables, such as MnO2 dose (0–15 mg/L), KOH dose (0–1 mol/L), urea dose (0–1.2 mol/L), reaction time (30–150 min), and temperature (25–85 °C), were systematically explored to optimize AHA yield, carboxylic acid content, and lignin removal. Optimal conditions, employing 7.69 mg/L of MnO2, 0.57 mol/L of KOH, and 0.63 mol/L of urea at 85 °C for 106 min, resulted in an impressive AHA yield of 32.15%, featuring a significant carboxylic acid content of 3.325 mmol/g. Under these conditions, up to 65% of lignin was effectively removed, accompanied by the release of orthophosphate up to 258 mg/L. The produced AHAs exhibited reduced toxicity, as demonstrated by substantial reductions of 54.13%, 57.14%, and 42.50% in phenols, furfural, and hydroxymethylfurfural, respectively. Notably, the AHAs displayed favorable characteristics, including a lower molecular weight (760.49 g/mol), diminished aromaticity (66.25% reduction), higher humification degree (lower C/N ratio of 8.79), increased oxidation degree (higher O/C ratio of 0.6), and elevated spectral index of humification (higher E4/E6 of 4.58). Analytical techniques, such as FT-IR, XPS, and TGA-MS, revealed chemical resemblance and enhanced functionality of AHAs compared to natural counterparts. Differentiation between AHA, lignin, and humification residues was confirmed through SEM-EDX, ICP-OES, and organic/inorganic carbon analyses. The obtained AHAs exhibit promising characteristics suitable for diverse applications in sustainable agriculture and environmental management. 

Oxidation-alkaline-enhanced abiotic humification valorizes lignin-rich biogas digestate into artificial humic acids

Regulating pH and Phanerochaete chrysosporium inoculation improved the humification and succession of fungal community at the cooling stage of composting.

A green process for producing xylooligosaccharides via autohydrolysis of plasma-treated sugarcane bagasse

Phosphorus transformation behavior and phosphorus cycling genes expression in food waste composting with hydroxyapatite enhanced by phosphate-solubilizing bacteria.

Highly digestible nitrogen-enriched straw upgraded by ozone-urea pretreatment: digestibility metrics and energy-economic analysis.

Bauhinia variegata petals are colorful, rich in anthocyanins, and have ornamental, nutritional, and medicinal value. However, the regulatory mechanism of anthocyanin accumulation in B. variegata remains unclear. In this study, a combined analysis of the metabolome and transcriptome was performed in red and white B. variegata cultivars in the early, middle, and blooming stages. A total of 46 different anthocyanins were identified, of which 27 showed marked differences in accumulation between the two cultivars, and contribute to their different petal colors. Malvidin 3-O-galactoside, peonidin 3-O-galactoside, cyanidin 3-O-glucoside, cyanidin 3-O-galactoside, and malvidin 3-O-glucoside were much more abundant in the second stage of flowering. In the blooming stage, except for the anthocyanins mentioned, delphinidin 3-O-galactoside and petunidin 3-O-galactoside were the most abundant anthocyanins in the red flowers, indicating that malvidin, peonidin, cyanidin, delphinidin, and petunidin were all responsible for the red color of petals in B. variegata. RNA sequencing identified 2,431 differentially expressed genes (DEGs), of which 26 were involved in the anthocyanin synthesis pathway. Correlations between the anthocyanin biosynthesis-related DEGs and anthocyanin contents were explored, and the DEGs involved in anthocyanin accumulation in B. variegata petals were identified. Eighteen of these DEGs encoded key catalytic enzymes, such as anthocyanidin reductase (ANR) and flavonoid-3′5′-hydroxylase (F3′5′H), and 17 of them encoded transcription factors (TFs) belonging to 14 families (including MYB, NAC, SPL, ERF, and CHR28). These results improve our understanding of the roles of anthocyanins, catalytic enzymes, and TFs in B. variegata petal-color expression.

/pdf/combined-analysis-of-the-transcriptome-and-metabolome-dxz87tar.pdf

Combined Analysis of the Transcriptome and Metabolome Revealed the Mechanism of Petal Coloration in Bauhinia variegata

Abstract Cercidoideae, one of the six subfamilies of Leguminosae, contains one genus Cercis with its chromosome number 2n = 14 and all other genera with 2n = 28. An allotetraploid origin hypothesis for the common ancestor of non-Cercis genera in this subfamily has been proposed; however, no chromosome-level genomes from Cercidoideae have been available to test this hypothesis. Here, we conducted a chromosome-level genome assembly of Bauhinia variegata to test this hypothesis. The assembled genome is 326.4 Mb with the scaffold N50 of 22.1 Mb and contains 37,996 protein-coding genes. The Ks distribution between gene pairs in the syntenic regions indicates two whole-genome duplications (WGDs): one is B. variegata-specific, and the other is shared among core eudicots. Although Ks between gene pairs generated by the recent WGD in Bauhinia is greater than that between Bauhinia and Cercis, the WGD was not detected in Cercis, which can be explained by an accelerated evolutionary rate in Bauhinia after divergence from Cercis. Ks distribution and phylogenetic analysis for gene pairs generated by the recent WGD in Bauhinia and their corresponding orthologs in Cercis support the allopolyploidy origin hypothesis of Bauhinia. The genome of B. variegata also provides a genomic resource for dissecting genetic basis of its ornamental traits.

Chromosomal-level genome assembly of the orchid tree Bauhinia variegata (Leguminosae; Cercidoideae) supports the allotetraploid origin hypothesis of Bauhinia

l.Black Soybeans (Glycine max (L.) Merr.) is a rare legume native for agricultural production by clearing toxins from the body in Chinese medicine. In this study, the chloroplast genome of G. max (...

Characterization of the complete chloroplast genome of black soybeans, Glycine max (L.) Merr. (legume)

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