Da-Yuan Wang
GlaxoSmithKline
7 Papers
58 Citations
Da-Yuan Wang is an academic researcher from GlaxoSmithKline. The author has contributed to research in topics: BacMam & Expression cassette. The author has an hindex of 5, co-authored 6 publications.
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Papers
Discovery of potent, selective sulfonylfuran urea endothelial lipase inhibitors.
Krista B. Goodman,Michael Jonathan Bury,Mui Cheung,Maria Cichy-Knight,Sarah E. Dowdell,Allison K. Dunn,Dennis Lee,Jeffrey A. Lieby,Michael L. Moore,Daryl A. Scherzer,Deyou Sha,Dominic Suarez,Dennis Murphy,Mark R. Harpel,Eric S. Manas,Dean E. McNulty,Roland S. Annan,Rosalie Matico,Benjamin K. Schwartz,John J. Trill,Thomas D. Sweitzer,Da-Yuan Wang,Paul M. Keller,John A. Krawiec,Michael Jaye +24 more
TL;DR: Sulfonylfuran urea 1 was identified in a high-throughput screening campaign as a potent and non-selective EL inhibitor, and modifications leading to improved LPL selectivity were identified.
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Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
TL;DR: This chapter highlights the BacMam transfer vector pHTBV, which has become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide.
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Development of a High-Throughput Screen to Detect Inhibitors of TRPS1 Sumoylation
Martin Brandt,Lawrence M. Szewczuk,Hong Zhang,Xuan Hong,Patricia M. McCormick,Tia Lewis,Taylor I Graham,Sunny T Hung,Amber D Harper-Jones,John J. Kerrigan,Da-Yuan Wang,Edward Dul,Wangfang Hou,Thau F. Ho,Thomas D. Meek,Mui H Cheung,Kyung O. Johanson,Christopher S. Jones,Benjamin J. Schwartz,Sanjay Kumar,Allen Oliff,Robert B. Kirkpatrick +21 more
TL;DR: A high-throughput fluorescence polarization assay was developed, which allows detection of Lys-1201 sumoylation, corresponding to the major site of functional sumoylated within the transcriptional repressor trichorhino-phalangeal syndrome type I protein (TRPS1).
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Development of a High-Throughput Cell-Based Assay for 11β-Hydroxysteroid Dehydrogenase Type 1 Using BacMam Technology
Da-Yuan Wang,Quinn Lu,Stacey L. Walsh,Lisa A. Payne,Sundip Modha,Martin J. Scott,Thomas D. Sweitzer,Robert S. Ames,Daniel J. Krosky,Hu Li +9 more
TL;DR: A sensitive and robust high-throughput cell-based assay for screening 11β-HSD1 inhibitors and it is shown that co-expression of hexose-6-phosphate dehydrogenase (H6PDH) is not required for the enzyme to function effectively as an oxo-reductase.
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Assay development and high-throughput screening of small molecular c-Abl kinase activators.
Josh Cottom,Glenn A. Hofmann,Brett Siegfried,Jingsong Yang,Hong Zhang,Tracey Yi,Thau F. Ho,Chad Quinn,Da-Yuan Wang,Kyung O. Johanson,Robert S. Ames,Hu Li +11 more
TL;DR: A 2-step kinase assay was developed and used in a high-throughput screen (HTS) of more than 1 million compounds in an effort to identify c-Abl tyrosine kinase activators and selective hits were shown to be cell permeable and were able to induce c- abl activation as determined by In-Cell Western analysis.
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