Negative Effects of a High-Fat Diet on Intestinal Permeability: A Review

Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the most versatile in terms of long-term tracking of lymphocytes and their ability to quantify lymphocyte proliferation. They are the intracellular covalent coupling dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and the membrane inserting dye PKH26. Both dyes have the advantage that they can be used to track cell division, both in vitro and in vivo, due to the progressive halving of the fluorescence intensity of the dyes in cells after each division. However, CFSE appears to have the edge over PKH26 based on homogeneity of lymphocyte staining and cost. Two other fluorescent dyes, although not suitable for lymphocyte proliferation studies, are valuable tracking dyes for short-term (up to 3 day) lymphocyte migration experiments, namely the DNA-binding dye Hoechst 33342 and the cytoplasmic dye calcein. In the future it is highly likely that additional fluorescent dyes, with different spectral properties to CFSE, will become available, as well as membrane inserting fluorescent dyes that more homogeneously label lymphocytes than PKH26.

Fluorescent dyes for lymphocyte migration and proliferation studies

https://www.gastrojournal.org/article/S0016-5085(98)70328-2/pdf

Leukocyte-endothelial cell interactions: Molecular mechanisms and implications in gastrointestinal disease

The endothelin system in septic and endotoxin shock.

A chronic inflammatory disease is a condition characterized by persistent inflammation. A number of human pathologies fall into this category, and a great deal of research has been conducted to learn more about their characteristics and underlying mechanisms. In many cases, a genetic component has been identified, but also external factors like food, smoke, or environmental pollutants can significantly contribute to worsen their symptoms. Accumulated evidence clearly shows that chronic inflammatory diseases are subjected to a redox control. Here, we shall review the identity, source, regulation, and biological activity of redox molecules, to put in a better perspective their key-role in cancer, diabetes, cardiovascular diseases, atherosclerosis, chronic obstructive pulmonary diseases, and inflammatory bowel diseases. In addition, the impact of redox species on autoimmune disorders (rheumatoid arthritis, systemic lupus erythematosus, psoriasis, and celiac disease) and neurodegenerative diseases (A...

Chronic inflammatory disorders and their redox control: from molecular mechanisms to therapeutic opportunities.

Although lymphocyte-endothelial cell interactions represent a key step in controlling the recruitment of lymphocytes into gut-associated tissues, its dynamic process in microvessels of lymphoid (Pe...

Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa

https://www.gastrojournal.org/article/S0016-5085(97)00120-0/pdf

Enhanced Lymphocyte Interaction in Postcapillary Venules of Peyer's Patches During Fat Absorption in Rats

https://www.gastrojournal.org/article/S0016-5085(98)70141-6/pdf

Nitric oxide modulates T-lymphocyte migration in Peyer's patches and villous submucosa of rat small intestine

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Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa

Enhanced Lymphocyte Interaction in Postcapillary Venules of Peyer's Patches During Fat Absorption in Rats

Nitric oxide modulates T-lymphocyte migration in Peyer's patches and villous submucosa of rat small intestine