A benzo BODIPY based fluorescent probe for selective visualization of hypochlorous acid in living cells and zebrafish.

Hypochlorous acid (HOCl) is a common reactive oxygen species (ROS) associated with the development of liver, tumor, inflammatory, and other diseases. In this work, the turn-on fluorescent probe named (WZ-HOCl) with a naphthalimide structure was designed and synthesized to detect endogenous HOCl in disease models. WZ-HOCl can achieve a fast response to HOCl with good linearity in the range of 0–45 μM (LOD = 147 nM). The application of WZ-HOCl in bioimaging was investigated by constructing a series of cellular disease models, and the results showed that WZ-HOCl could sensitively detect endogenous HOCl in inflammatory and liver disease models. It can also be used to differentiate between hepatocytes and hepatoma cells. WZ-HOCl will provide new methods and ideas for fluorescent probes in detecting drug-induced liver injury, alcoholic and non-alcoholic steatohepatitis, and some inflammation-related diseases. 

A turn-on fluorescent probe for detecting and bioimaging of HOCl in inflammatory and liver disease models.

The endoplasmic reticulum (ER) is one of the most important organelles in cells, involved in protein synthesis, folding, and modification, as well as Ca2+ storage and release. ER homeostasis imbalances may contribute to various diseases, including neurodegenerative diseases, cardiovascular diseases, metabolic diseases, and cancer. Current research efforts primarily focus on ER-related disease mechanisms and drug candidate screening. Because disease processes involve multiple molecular events and signaling, an innovative tool is necessary to precisely detect key signaling molecules in the ER to accelerate the discovery of targeted therapeutics. In recent years, because of their real-time visualization, precise targeting, high sensitivity and specificity, low cytotoxicity, and synthetic tunability, fluorescent probes have been widely used to investigate biological processes and disease events. This review first introduces ER and its related biological processes, and the design rules of ER-targeted probes. Subsequently, based on the latest literature, it systematically summarizes the research progress of fluorescent probes for imaging physiological or pathological events in the ER. Lastly, it discusses the challenges of ER-targeted probes as well as the future development of probe engineering. We anticipate that this review will not only encourage the development of fluorescence probe engineering but will also enhance interdisciplinary research between chemistry, biology, pharmacology, and medicine, promoting for its widespread application. 

New tool for diseases mechanism studies: Endoplasmic reticulum-targeted fluorescent probes

A phosphine-based fluorescent probe was developed for rapid and sensitive detection of HOCl in living cells and zebrafish.

A phosphine-based fluorescent probe for fluorescent imaging of hypochlorous acid in living cells and zebrafish

Reaction-based small-molecule fluorescent probes for endoplasmic reticulum- and mitochondria-targeted biosensing and bioimaging

Probing drug-mediated fluctuations of HClO levels in the endoplasmic reticulum by a ratiometric fluorescent probe with a large emission shift

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Probing drug-mediated fluctuations of HClO levels in the endoplasmic reticulum by a ratiometric fluorescent probe with a large emission shift