Protein deposition on contact lenses: the past, the present, and the future.

AFM and SFG studies of pHEMA-based hydrogel contact lens surfaces in saline solution: adhesion, friction, and the presence of non-crosslinked polymer chains at the surface

Biofouling on medical devices generally causes adverse complications, such as thrombosis, infection, and pathogenic calcification. Silicone is a widely used material for medical applications. Its surface modification typically encounters undesirable “hydrophobic recovery”, leading to deterioration of surface engineering. In this study, we developed a stable superhydrophilic zwitterionic interface on polydimethylsiloxane (PDMS) elastomer by covalent silanization of sulfobetaine silane (SBSi) to resist nonspecific adsorption of bacteria, proteins, and lipids. SBSi is a zwitterionic organosilane assembly, enabling resisting surface reconstruction by forming a cross-linked network and polar segregation. Surface elemental composition was confirmed by X-ray photoelectron spectroscopy (XPS), and the long-term stability of modification was accessed using a contact angle goniometer. The biofouling tests were carried out by exposing substrates to bacterial, protein, and lipid solutions, revealing the excellent bioi...

Modification of Silicone Elastomer with Zwitterionic Silane for Durable Antifouling Properties

We sought to compare the kinetics of in vitro lysozyme deposition on silicone hydrogel (SH), polymethyl methacrylate (PMMA), and FDA groups I, II, and IV contact lenses. Lenses were incubated in 125I-labeled lysozyme for time periods ranging from 1 hr to 28 days, and radioactive counts were determined. SH lenses and PMMA deposited less lysozyme than conventional hydrogel lenses (p < 0.05). Lysozyme accumulation on group IV lenses reached a maximum on the seventh day and then plateaued, whereas on groups I, II, and SH lenses, deposition continued to increase across all time periods, reiterating that kinetics of lysozyme deposition is highly material dependent.

Kinetics of in vitro lysozyme deposition on silicone hydrogel, PMMA, and FDA groups I, II, and IV contact lens materials.

PURPOSE An in vitro study was conducted to measure the adsorption of major tear film lipids to soft contact lenses over time. METHODS Commercial balafilcon A (PureVision; Bausch & Lomb, Rochester, NY), galyfilcon A (Acuvue Advance; Johnson & Johnson Vision Care), lotrafilcon A and B (Night & Day And O(2)Optix; CIBA Vision, Duluth, GA), senofilcon A (Acuvue Oasys; Johnson & Johnson Vision Care, Jacksonville, FL) and etafilcon A (Acuvue 2; Johnson & Johnson Vision Care) lenses were all soaked for 14 hours in the dark at 34.5 degrees C in either cholesterol (CH; nonpolar lipid) or phosphatidylethanolamine (PE; polar lipid), tagged with 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) and N-fluorescein-5-thiocarbamoyl (FITC) labels, respectively. After rinsing, the lenses were measured for fluorescence and the corresponding lipid concentration was calculated from an appropriate standard curve. The lenses were then placed into a fresh 1-mL aliquot of the lipid being tested, and the procedure was repeated for 20 days. RESULTS In vitro adsorption of CH was greater that that of PE for all lens types (P < 0.0001 at days 14 and 20). After 20 days of soaking in PE, the lotrafilcon polymers showed the lowest adsorption of all the silicone hydrogel lenses tested at 0.4 and 1.5 microg/lens, for lotrafilcon A (P < or = 0.0001) and lotrafilcon B, respectively (P < or = 0.0001). Galyfilcon A and senofilcon A showed significantly higher PE adsorption at 5.1 and 4.9 microg/lens, respectively, compared with all other silicone hydrogel lenses investigated (P < 0.03). Senofilcon A (P < 0.0001) and balafilcon A (P < 0.02) had the highest affinity for CH of all the lens types after 20 days, with adsorptions of 23.2 and 24.1 microg/lens, respectively. Lotrafilcon B (P < 0.0001) showed the lowest in vitro adsorption of CH of all the lens types, at 3 mug/lens. CONCLUSIONS In vitro lipid adsorption varied greatly depending on the lens material for both polar and nonpolar lipids. Overall, there was less in vitro adsorption of lipid to the lotrafilcon A and B polymers than for any of the other silicone hydrogel polymers tested. The quantity of lipid adsorption by lotrafilcon polymers was similar to "conventional" hydrogel lenses.

/pdf/the-adsorption-of-major-tear-film-lipids-in-vitro-to-various-52kmfdzbqw.pdf

The Adsorption of Major Tear Film Lipids In Vitro to Various Silicone Hydrogels over Time

Artificial tear adsorption on soft contact lenses: methods to test surfactant efficacy

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Artificial tear adsorption on soft contact lenses: methods to test surfactant efficacy