Cuilan Zhou

TL;DR: In this article, qPCR targeting the arithmetic-logic unit (Alu) repeats were performed using two different sets of primers amplifying the long and short segments, and DNA integrity was calculated by the ratio of the long to the short fragments of amplified Alu repeats.

TL;DR: In this paper, a primer pair for detecting hot-spot mutation of tumor mutant KRAS genes at the amino acid codons 12 and 13 was provided, where the principle of in-vitro adjustment of the reading frame of genes is adopted, and the blue-white screening for sub-cloning is used for detecting the hot spot mutation.

TL;DR: In this article , a mitochromic analysis using Neanderthal mtDNA as a foreign transgene and humans as a naturally occurring transgenic species was performed. But the results showed that the mtDNA retrieved from Neanderthal fossils and mtDNA of early modern human fossils dating back to approximately the same time in the Pleistocene constitutes a limitation that may compromise the significance of mtDNA phylogenetic analysis.

TL;DR: In this paper, a detection method of gene hot-spot mutation was proposed, by conducting restriction enzyme digestion on a wild sequence in vitro and by conducting treatment, which is not good for a ligation reaction, at the end of an incision, sub-clone an in-vitro amplification fragment, and then implements sequencing analysis on a colony formed by cloning.