Cornelia Hunke
Nanyang Technological University
25 Papers
237 Citations
Cornelia Hunke is an academic researcher from Nanyang Technological University. The author has contributed to research in topics: Protein subunit & ATP synthase. The author has an hindex of 10, co-authored 25 publications.
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Papers
Crystal structure of the NS3 protease-helicase from dengue virus
Dahai Luo,T. Xu,Cornelia Hunke,Gerhard Grüber,Subhash G. Vasudevan,Julien Lescar,Julien Lescar +6 more
TL;DR: It is proposed that a basic patch located at the surface of the protease domain increases the affinity for nucleotides and could also participate in RNA binding, explaining the higher unwinding activity of the full-length enzyme compared to that of the isolated helicase domain.
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Crystal structure of the NS3 protease-helicase from dengue virus
Dahai Luo,T. Xu,Cornelia Hunke,Gerhard Grüber,Subhash G. Vasudevan,Julien Lescar,Julien Lescar +6 more
TL;DR: In this article, the authors reported a crystallographic structure of a complete NS3 molecule fused to 18 residues of the NS2B cofactor at a resolution of 3.15 A. The relative orientation between the protease and helicase domains is drastically different than the single-chain NS3-NS4A molecule from hepatitis C virus, which was caught in the act of cis cleavage at the NS3 and NS4A junction.
Cloning, purification, and nucleotide-binding traits of the catalytic subunit A of the V1VO ATPase from Aedes albopictus.
TL;DR: Tryptophan fluorescence quenching of subunit A after binding of different nucleotides provides evidence for secondary structural alterations in this subunit caused by nucleotide-binding, and Quantitation of the FCS data indicates that the ADP-analogues bind slightly weaker to sub unit A than the ATP- analogues.
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Spectroscopic and crystallographic studies of the mutant R416W give insight into the nucleotide binding traits of subunit B of the A1Ao ATP synthase.
Anil Kumar,Malathy Sony Subramanian Manimekalai,Asha Manikkoth Balakrishna,Cornelia Hunke,Sven Weigelt,Norbert Sewald,Gerhard Grüber +6 more
TL;DR: The trapped ATP position, similar to the one of the binding region of the antibiotic efrapeptin in F1FO ATP synthases, is discussed in light of a transition nucleotide‐binding state of ATP while on its way to the final binding pocket.
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ATP/ADP Binding to a Novel Nucleotide Binding Domain of the Reticulocyte-binding Protein Py235 of Plasmodium yoelii
TL;DR: The data suggest that different structural changes induced by ATP/ADP binding to RBL could play an important role during the invasion process.
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