Clare E. Sample

TL;DR: Using transient transfection assays, it is demonstrated that EBNA-3C has two effects on reporter genes that are linked to the latent membrane protein 1 promoter, low-level activation by EBNA -3C alone, as well as potentiation ofEBNA-2-mediated transactivation, and inhibition of the normally strong activation mediated by EB NA-2.

TL;DR: It is demonstrated that EBV is able to infect organotypic cultures of epithelial cells to establish a predominantly productive infection in the suprabasal layers of stratified epithelium, similar to that seen with Kaposi’s-associated herpesvirus.

TL;DR: The ability of the EBNA-3 proteins to prevent Jkappa from binding to DNA in vitro and suppress transactivation via Jkappas DNA elements suggests that theEBNA- 3 proteins act analogously to the Drosophila protein Hairless.

TL;DR: Identification of defective, integrated viral DNA in some tumors indicates greater involvement of virus in sporadic Burkitt's lymphoma than previously documented and suggests a process of viral DNA rearrangement and loss during malignant progression most consistent with an initiating role for EBV in tumorigenesis.