Christopher J. Hoyle
University of Leeds
5 Papers
66 Citations
Christopher J. Hoyle is an academic researcher from University of Leeds. The author has contributed to research in topics: Membrane transport protein & Membrane protein. The author has an hindex of 5, co-authored 5 publications. Previous affiliations of Christopher J. Hoyle include GlaxoSmithKline.
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Papers
Microbial Drug Efflux Proteins of the Major Facilitator Superfamily
Massoud Saidijam,Giulia Benedetti,Qinghu Ren,Zhiqiang Xu,Christopher J. Hoyle,S.L. Palmer,Alison Ward,Kim E. Bettaney,Gerda Szakonyi,Johan Meuller,Scott Morrison,Martin K Pos,Patrick Butaye,Karl Walravens,Kate P. Langton,Richard B. Herbert,Ronald A. Skurray,Ronald A. Skurray,Ian T. Paulsen,John O'Reilly,Nicholas G. Rutherford,Melissa H. Brown,Melissa H. Brown,Roslyn M. Bill,Peter J. F. Henderson +24 more
TL;DR: A structural genomics approach for the amplified expression, purification and characterisation of prokaryotic drug efflux proteins of the 'Major Facilitator Superfamily' of transport proteins from Helicobacter pylori, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Bacillus subtilis, Brucella melitensis, Campylobacter jejuni and Neisseria meningitides is described.
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•Journal Article
Prokaryote Multidrug Efflux Proteins of the Major Facilitator Superfamily: Amplified Expression, Purification and Characterisation
Alison Ward,Christopher J. Hoyle,S.L. Palmer,John O'Reilly,Jeffrey Griffith,Martin K Pos,Scott Morrison,Berend Poolman,M. Gwynne,Peter J. F. Henderson +9 more
TL;DR: A general strategy for the amplified expression, purification and characterisation of prokaryotic multidrug efflux proteins of the 'Major facilitator superfamily' of transport proteins is described, using the Bacillus subtilisMultidrug resistance protein, 'Bmr', as example.
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Cloning, expression, purification and properties of a putative multidrug resistance efflux protein from Helicobacter pylori
TL;DR: Cl cloning and expression in Escherichia coli of the H. pylori gene hp1181 encoding a putative multidrug resistance membrane transport protein is described, and the retention of structural integrity and occurrence of predicted alpha-helix in the protein were verified by circular dichroism spectroscopy.
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A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily
Gerda Szakonyi,Dong Leng,Pikyee Ma,Kim E. Bettaney,Massoud Saidijam,Alison Ward,Saeid Zibaei,Alastair T. Gardiner,Richard J. Cogdell,Patrick Butaye,Anne-Brit Kolstø,John O'Reilly,Ryan J. Hope,Nicholas G. Rutherford,Christopher J. Hoyle,Peter J. F. Henderson +15 more
TL;DR: A genomic strategy for the overexpression of bacterial multidrug and antibiotic resistance membrane efflux proteins in Escherichia coli is described and encoded proteins from a range of Gram-positive and Gram-negative bacteria comprise 5% to 35% of E. coli inner membrane protein.
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Collection and characterisation of bacterial membrane proteins
Massoud Saidijam,Georgios Psakis,Joanne Clough,Johan Meuller,Shunichi Suzuki,Christopher J. Hoyle,S.L. Palmer,Scott Morrison,Martin K Pos,Richard C. Essenberg,Martin C. J. Maiden,Atif A.W. Abu‐Bakr,Simon Baumberg,Alex A Neyfakh,Jeffrey K Griffith,Michael J. R. Stark,Alison Ward,John O'Reilly,Nicholas G. Rutherford,Mary K. Phillips-Jones,Peter J. F. Henderson +20 more
TL;DR: A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described and this strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from otheracteria.