Christopher B. Eiben
University of California, Berkeley
12 Papers
27 Citations
Christopher B. Eiben is an academic researcher from University of California, Berkeley. The author has contributed to research in topics: Pseudomonas putida & Metabolic pathway. The author has an hindex of 7, co-authored 12 publications. Previous affiliations of Christopher B. Eiben include Lawrence Berkeley National Laboratory & University of California, San Francisco.
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Papers
Computational protein design enables a novel one-carbon assimilation pathway
Justin B. Siegel,Amanda L. Smith,Sean Poust,Adam J. Wargacki,Arren Bar-Even,Catherine Louw,Betty W. Shen,Christopher B. Eiben,Huu M. Tran,Huu M. Tran,Elad Noor,Jasmine L. Gallaher,Jacob B. Bale,Yasuo Yoshikuni,Yasuo Yoshikuni,Michael H. Gelb,Jay D. Keasling,Barry L. Stoddard,Mary E. Lidstrom,David Baker +19 more
TL;DR: A computationally designed enzyme, formolase (FLS), which catalyzes the carboligation of three one-carbon formaldehyde molecules into one three-carbon dihydroxyacetone molecule is described, demonstrating how modern protein engineering and design tools can facilitate the construction of a completely new biosynthetic pathway.
Mevalonate Pathway Promiscuity Enables Noncanonical Terpene Production.
Christopher B. Eiben,Tristan de Rond,Clayton S. Bloszies,Jennifer W. Gin,Jennifer W. Gin,Jennifer Chiniquy,Jennifer Chiniquy,Edward E. K. Baidoo,Edward E. K. Baidoo,Christopher J. Petzold,Christopher J. Petzold,Nathan J. Hillson,Nathan J. Hillson,Oliver Fiehn,Jay D. Keasling +14 more
TL;DR: Heterologously expressed the lepidopteran modified mevalonate pathway, a propionyl-CoA ligase, and terpene cyclases in E. coli to produce several novel terpenes containing 16 carbons, which should greatly expand the reachable biochemical space with applications in areas where Terpenes have traditionally found uses.
An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism
Mitchell G. Thompson,Mitchell G. Thompson,Mitchell G. Thompson,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jacquelyn M. Blake-Hedges,Jose Henrique Pereira,Jose Henrique Pereira,John A. Hangasky,Michael S. Belcher,Michael S. Belcher,Michael S. Belcher,William M. Moore,William M. Moore,William M. Moore,Jesus F. Barajas,Jesus F. Barajas,Pablo Cruz-Morales,Pablo Cruz-Morales,Lorenzo J. Washington,Lorenzo J. Washington,Lorenzo J. Washington,Robert W. Haushalter,Robert W. Haushalter,Christopher B. Eiben,Christopher B. Eiben,Christopher B. Eiben,Yuzhong Liu,Yuzhong Liu,Will Skyrud,Veronica T. Benites,Veronica T. Benites,Tyler P. Barnum,Edward E. K. Baidoo,Edward E. K. Baidoo,Henrik Vibe Scheller,Henrik Vibe Scheller,Henrik Vibe Scheller,Michael A. Marletta,Patrick M. Shih,Paul D. Adams,Paul D. Adams,Paul D. Adams,Jay D. Keasling +43 more
TL;DR: Structural and biochemical approaches are used to show that HglS acts via successive decarboxylation and intramolecular hydroxylation in a Fe(II)- and O 2 -dependent manner and that homologous enzymes catalyze the final step of lysine catabolism in plants.
Improvement of a potential anthrax therapeutic by computational protein design
Sean J. Wu,Christopher B. Eiben,John H. Carra,Ivan Huang,David M. Zong,Peixian Liu,Cindy T. Wu,Jeff Nivala,Josef A. Dunbar,Tomas Huber,Jeffrey L. Senft,Rowena D Schokman,Matthew D. Smith,Jeremy H. Mills,Arthur M. Friedlander,David Baker,Justin B. Siegel +16 more
TL;DR: This study shows that circular permutation of CapD improved production properties and dramatically increased kinetic thermostability, and identifies an amino acid substitution that dramatically decreased transpeptidation activity but not hydrolysis.
12
Adenosine Triphosphate and Carbon Efficient Route to Second Generation Biofuel Isopentanol.
Christopher B. Eiben,Christopher B. Eiben,Tian Tian,Tian Tian,Mitchell G. Thompson,Mitchell G. Thompson,Mitchell G. Thompson,Daniel Mendez-Perez,Daniel Mendez-Perez,Nurgul Kaplan,Nurgul Kaplan,Garima Goyal,Garima Goyal,Jennifer Chiniquy,Jennifer Chiniquy,Nathan J. Hillson,Nathan J. Hillson,Taek Soon Lee,Taek Soon Lee,Jay D. Keasling +19 more
TL;DR: A pathway from the renewable resource glucose to next generation biofuel isopentanol is demonstrated by pairing the isovaleryl- CoA biosynthesis pathway from Myxococcus xanthus and a butyryl-CoA reductase from Clostridium acetobutylicum with the best plasmid and Escherichia coli strain combination.