Christopher Acree
Columbia University
9 Papers
6 Citations
Christopher Acree is an academic researcher from Columbia University. The author has contributed to research in topics: Transposase & Transposable element. The author has an hindex of 3, co-authored 4 publications.
Chat about Author
Papers
CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering.
Phuc Leo H. Vo,Carlotta Ronda,Sanne E. Klompe,Ethan E. Chen,Christopher Acree,Harris H. Wang,Samuel H. Sternberg +6 more
TL;DR: A substantially improved version of the Tn7-like transposon from Vibrio cholerae is introduced, which uses a Type I-F CRISPR-Cas system for programmable, RNA-guided transposition and establishes INTEGRATE as a versatile tool for multiplexed, kilobase-scale genome engineering.
CRISPR RNA-guided integrases for high-efficiency and multiplexed bacterial genome engineering
Phuc Leo H. Vo,Carlotta Ronda,Sanne E. Klompe,Ethan E. Chen,Christopher Acree,Harris H. Wang,Samuel H. Sternberg +6 more
TL;DR: This work establishes INTEGRATE as a versatile and portable tool that enables multiplex and kilobase-scale genome engineering and develops an accessible computational algorithm for guide RNA design.
Mechanism of target site selection by type V-K CRISPR-associated transposases
Jerrin Thomas George,Christopher Acree,Jung-Un Park,Muwen Kong,Tanner Wiegand,Yanis Luca Pignot,Elizabeth H. Kellogg,Eric C. Greene,Samuel H. Sternberg +8 more
TL;DR: It is revealed that type V-K CASTs maintain an RNA-independent, “untargeted” transposition pathway alongside RNA-dependent integration, driven by the local availability of TnsC filaments.
12
Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing.
TL;DR: In this article, the authors apply long-read, population-based whole-genome sequencing (WGS) to unambiguously resolve transposition products for two evolutionarily distinct transposon types that employ either Cascade or Cas12k for RNA-guided DNA integration.