Christophe Fremaux
DuPont
65 Papers
434 Citations
Christophe Fremaux is an academic researcher from DuPont. The author has contributed to research in topics: Streptococcus thermophilus & CRISPR. The author has an hindex of 25, co-authored 62 publications. Previous affiliations of Christophe Fremaux include Laval University & Vilnius University.
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Papers
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA.
Josiane E. Garneau,Marie Ève Dupuis,Manuela Villion,Dennis A. Romero,Rodolphe Barrangou,Patrick Boyaval,Christophe Fremaux,Philippe Horvath,Alfonso H. Magadán,Sylvain Moineau +9 more
TL;DR: In vivo evidence is provided that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading toplasmid loss.
Phage response to CRISPR-encoded resistance in Streptococcus thermophilus.
Hélène Deveau,Rodolphe Barrangou,Josiane E. Garneau,Jessica M. Labonté,Christophe Fremaux,Patrick Boyaval,Dennis A. Romero,Philippe Horvath,Sylvain Moineau +8 more
TL;DR: It is shown through the analyses of 20 mutant phages that virulent phages are rapidly evolving through single nucleotide mutations as well as deletions, in response to CRISPR1.
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The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
Rimantas Sapranauskas,Giedrius Gasiunas,Christophe Fremaux,Rodolphe Barrangou,Philippe Horvath,Virginijus Siksnys +5 more
TL;DR: The results show that active CRISPR/Cas systems can be transferred across distant genera and provide heterologous interference against invasive nucleic acids and can be leveraged to develop strains more robust against phage attack, and safer organisms less likely to uptake and disseminate plasmid-encoded undesirable genetic elements.
Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus
Philippe Horvath,Dennis A. Romero,Anne Claire Coûté-Monvoisin,Melissa Richards,Hélène Deveau,Sylvain Moineau,Patrick Boyaval,Christophe Fremaux,Rodolphe Barrangou +8 more
TL;DR: A novel Streptococcus thermophilus locus is characterized, and its ability to integrate novel spacers in response to bacteriophage is experimentally demonstrated, and it is hypothesized that the level of diversity is correlated with relative CRISPR activity and proposed that the activity is highest for CRISpr1, followed by CRIS PR3, while CRISP2 may be degenerate.
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