Christine Milcarek
University of Pittsburgh
48 Papers
581 Citations
Christine Milcarek is an academic researcher from University of Pittsburgh. The author has contributed to research in topics: Polyadenylation & Messenger RNA. The author has an hindex of 24, co-authored 48 publications.
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Papers
Alternative poly(A) site selection in complex transcription units: means to an end?
TL;DR: The differential expression of a number of genes that undergo alternative poly(A) site choice or polyadenylation/splicing competition could be regulated at the level of amounts and activities of either generic or tissue-specific polyadenyation factors and/or splicing factors.
A comparison of apparent mRNA half-life using kinetic labeling techniques vs decay following administration of transcriptional inhibitors.
TL;DR: Several different techniques were used to determine the apparent half-lives of immunoglobulin gamma 2b heavy chain and kappa light chain mRNA's in mouse myeloma 4 T001 and a mutant derived from 4T001, i.e., mutant I17.
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Downstream sequence elements with different affinities for the hnRNP H/H′ protein influence the processing efficiency of mammalian polyadenylation signals
TL;DR: Using in vitro reconstitution assays, it is demonstrated that hnRNP H/H' can stimulate processing of two additional model polyadenylation signals by binding at similar relative downstream locations but with significantly different affinities.
Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition
Kathleen Martincic,Ronna L. Campbell,Gretchen Edwalds-Gilbert,Lina Souan,Michael T. Lotze,Christine Milcarek +5 more
TL;DR: The amount of the 64-kDa subunit of polyadenylation/cleavage stimulatory factor (CstF-64) increases 5-fold during the G0 to S phase transition and concomitant proliferation induced by serum in 3T6 fibroblasts and other lymphokine-induced factors play a role.
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HeLa cell poly(A)- mRNA codes for a subset of poly(A)+ mRNA-directed proteins with an actin as a major product.
TL;DR: A small number of poly(A)+ sequences appear to be present in relatively high concentration in poly (A)- mRNA as evidenced by both the translation products and the cross hybridization results.
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