Christian Leborgne
French Institute of Health and Medical Research
60 Papers
275 Citations
Christian Leborgne is an academic researcher from French Institute of Health and Medical Research. The author has contributed to research in topics: Transfection & Biology. The author has an hindex of 30, co-authored 58 publications. Previous affiliations of Christian Leborgne include Université Paris-Saclay & Institut Gustave Roussy.
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Papers
Prevalence of serum IgG and neutralizing factors against adeno-associated virus (AAV) types 1, 2, 5, 6, 8, and 9 in the healthy population: implications for gene therapy using AAV vectors.
Sylvie Boutin,Virginie Monteilhet,Philippe Veron,Christian Leborgne,Olivier Benveniste,Marie Montus,Carole Masurier +6 more
TL;DR: Characterization of the preexisting humoral responses to the AAV capsid and cross-reactivity will allow development of new strategies to circumvent AAV acquired immune responses, and vectors based on AAV5, AAV8, and AAV9 may have an advantage for gene therapy in humans.
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IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies
Christian Leborgne,Elena Barbon,Jeffrey M. Alexander,Hayley Hanby,Sandrine Delignat,Sandrine Delignat,Daniel M. Cohen,Fanny Collaud,Saghana Muraleetharan,Dan Lupo,Joseph Silverberg,Karen Huang,Laetitia van Wittengerghe,Béatrice Marolleau,Adeline Miranda,Anna Fabiano,Victoria Daventure,Victoria Daventure,Heena Beck,Xavier M. Anguela,Giuseppe Ronzitti,Sean M. Armour,Sébastien Lacroix-Desmazes,Sébastien Lacroix-Desmazes,Federico Mingozzi +24 more
TL;DR: Results provide a potential solution to overcome pre-existing antibodies to AAV-based gene therapy by showing efficient cleavage of pooled human IgG (intravenous Ig) in vitro upon endopeptidase treatment and reducing anti-AAV antibody levels from human plasma samples in vitro.
Synthesis of linear polyethylenimine derivatives for DNA transfection.
Blandine Brissault,Antoine Kichler,Christine Guis,Christian Leborgne,Olivier Danos,Hervé Cheradame +5 more
TL;DR: It is demonstrated that the high transfection efficiency of polyethylenimines does not solely rely on their capacity to capture protons which are transferred into the endo-lysosomes during acidification.
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Antigen-selective modulation of AAV immunogenicity with tolerogenic rapamycin nanoparticles enables successful vector re-administration
Amine Meliani,Amine Meliani,Florence Boisgerault,Romain Hardet,Solenne Marmier,Fanny Collaud,Giuseppe Ronzitti,Christian Leborgne,Helena Costa Verdera,Helena Costa Verdera,Marcelo Simon Sola,Marcelo Simon Sola,Severine Charles,Alban Vignaud,Laetitia van Wittenberghe,Giorgia Manni,Olivier D. Christophe,Francesca Fallarino,Christopher J. Roy,Alicia M. Michaud,Petr Ilyinskii,Takashi Kei Kishimoto,Federico Mingozzi,Federico Mingozzi +23 more
TL;DR: It is demonstrated that synthetic vaccine particles encapsulating rapamycin (SVP[Rapa], co-administered with AAV vectors, prevents the induction of anti-capsid humoral and cell-mediated responses, enabling efficient AAV redosing in mice and nonhuman primates.
Influence of Pre-existing Anti-capsid Neutralizing and Binding Antibodies on AAV Vector Transduction.
Zachary Fitzpatrick,Christian Leborgne,Elena Barbon,Elisa Masat,Giuseppe Ronzitti,Laetitia van Wittenberghe,Alban Vignaud,Fanny Collaud,Severine Charles,Marcelo Simon Sola,Fabienne Jouen,Olivier Boyer,Federico Mingozzi +12 more
TL;DR: AAV8 vectors administered to mice passively immunized with anti-AAV8 BAbs showed a more efficient liver transduction and a unique vector biodistribution profile compared to mice immunizing with NAbs, highlighting a virtually opposite effect of neutralizing and binding antibodies on AAV vectors transduction.
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