Chris Kitson

TL;DR: This study suggests that although siRNAs and asODNs can be developed to inhibit gene expression in culture systems and certain organs in vivo, barriers to nucleic acid transfer in airway epithelial cells seen with large DNA molecules may also affect the efficiency of in vivo uptake of smallucleic acid molecules.

TL;DR: The synthesis and associated structure-activity relationships for gene transfection of a series of spermine-derived cationic gemini surfactants incorporating diamino acid headgroups and either identical or different lipophilic tailgroups is described.

TL;DR: It is demonstrated that multivalent derivatization of a polymeric framework can create functional diversity substantially greater than the structural diversity of the derivatized building blocks and suggests an approach to a better understanding of the molecular underpinnings of transfection as well as their exploitation.

TL;DR: A cell-based oxidative stress-induced apoptosis assay is developed and a high-throughput screen (HTS) using a human druggable genome siRNA library is performed to identify potential novel pathways and targets that regulate the response of lung epithelial cells to oxidative stress.