The invention discloses a method for LAMP detection of enterohepatic hydatid of penaeus vannamei and a special kit for the method. The method comprises the steps of (1) collection of a penaeus vannamei sample; (2) extraction of a sample DNA; (3) preparation of an isothermal amplification system; (4) setting of an amplification device and an amplification program; and (5) result judgment. According to the method for LAMP detection of the enterohepatic hydatid of the penaeus vannamei, the efficiency and sensitivity of detection on the enterohepatic hydatid of the penaeus vannamei can be improved, effective monitoring of young or adult shrimp pathogens in the breeding process of the penaeus vannamei is ensured and the breeding risk is reduced; the dependence of enterohepatic hydatid detection on large expensive instruments is reduced and the fussy operation process of conventional PCR detection is simplified; the detection result can be identified through naked eyes and the result is straightforward; and aerosol pollution in amplification is avoided by adopting closed tube detection and result observation and the risk of a false positive result is reduced. The method has a good application prospect in monitoring of the enterohepatic hydatid of the penaeus vannamei.

Method for LAMP detection of enterohepatic hydatid of penaeus vannamei and special kit for method

The invention discloses a litopenaeus vannamei ecological breeding method based on live food and water quality bio-regulation. The method includes following steps: (1), treating water before breeding,and performing live food culture; (2), arranging larvae; (3), breeding, regulating water quality, and preventing and controlling pest and disease; (4), marking thickness, desalting, and transferring.An ecological breeding technique of chlamydomonas plus artemia plus probiotics plus compound Chinese herbal medicine is provided, and natural protein, trace elements and minerals are provided for theshrimp larvae, so that the shrimp larvae have strong physique and resistivity, and ecological shrimp larvae bred by the method are big in size, orderly in specification, quick in growth and high in survival rate. The breeding method is advanced in concept, scientific, reasonable, easy in implementation and suitable for large-scale application in high-health breeding of litopenaeus vannamei and has good social benefit and economic benefit.

Litopenaeus vannamei ecological breeding method based on live food and water quality bio-regulation

The invention discloses an epinephelus culture method. An outdoor ecological pond and an indoor fry breeding pond are connected through a pipeline, alga water in the outdoor ecological pond is pumpedinto the indoor fry breeding pond, a water discharge tube is arranged at the bottom of the indoor fry breeding pond and communicates with the outdoor ecological pond, tail water in the indoor fry breeding pond is discharged into the outdoor ecological pond, then a relay circulation epinephelus fry culture system with indoor fry culture and outdoor alga culture is formed, respective defects of ecological fry culture of an epinephelus pond and fry culture of factory-like circulation water are solved, advantages of the two fry breeding methods are taken into play sufficiently, SS type rotifera ofa sufficient density are provided as a starter feed for larva fishes starting to take feed at a starter stage, it ensures that most larva fishes can start to take feed smoothly at a first moment at the starter stage, live baits of a high density are continuously maintained at a larva fish development stage, all larva fishes can develop relatively synchronously at an early stage of fry breeding, individual differences of a same batch of fries can be reduced as much as possible, cannibalism can be avoided, the fry number can be greatly increased, and the fry rate can be increased.

Epinephelus culture method

The invention provides a method and device for culturing shrimps by adopting fully-closed circulating water. The method comprises the following steps of: directionally culturing microalgae and functional bacteria by adopting a multi-stage culture system, and converting edible algae and bacteria of filter-feeding aquatic organisms into animal proteins, wherein the number of algae cells is controlled to be 50,000 to 100,000/mL, and the number of bacteria cells is controlled to be 100,000 to 1,000,000/mL; and carrying out neutralization and sedimentation on culture water through porous mineral substances, and treating the culture water in a biofilter for recycling. The design is very standard and is favorable for modular replication, the land space is saved, and the culture efficiency is improved; in a circulating water system, the conversion path of organic wastes is fully considered, the water quality is directionally controlled through the microalgae and the functional bacteria, the efficiency of different conversion stages is coordinated, the filter-feeding aquatic organisms are introduced to utilize the bacteria and the algae in the culture water, algae proteins and bacterial proteins are converted into the animal proteins, and the culture cost is reduced to the greatest extent; and the method and device are reasonable in design, and the environment is protected on the premise of ensuring the culture benefit.

Method and device for culturing shrimps by adopting fully-closed circulating water

The invention discloses a Chinese herbal medicine composition capable of preventing and treating fish and shrimp white stool, and belongs to the technical field of seafood aquaculture The Chinese herbal medicine composition is prepared from, by weight, 8-12 parts of radix gentianae, 20-30 parts of isatis roots, 12-18 parts of folium isatidis, 6-10 parts of coptis chinensis, 4-6 parts of golden cypress, 6-10 parts of semen plantaginis, 4-6 parts of raix bupleuri, 4-6 parts of angelica sinensis, 8-12 parts of jasmine, 15-25 parts of common andrographis herb and 8-12 parts of scutellaria baicalensis When the Chinese herbal medicine composition is in use, Chinese herbal medicine is decocted into a decoction after being smashed and mixed with feed for feeding, the Chinese herbal medicine composition has the remarkable effects of enhancing the fish and shrimp immunity, preventing fish and shrimp morbidity and treating prawn play dead, white stool, jejunum, other diseases, fish enteritis and the other, and the Chinese herbal medicine has no side effect and can be used as health care medicine for feeding for a long term

Chinese herbal medicine composition capable of preventing and treating fish and shrimp white stool

The invention discloses an LAMP (Loop-mediated Isothermal Amplification) kit for a priB gene in an acute hepatopancreas necrosis syndrome and application thereof and belongs to the technical field of microbiological testing. The kit comprises an LAMP primer, a 2*reaction buffering solution, Bst DNA (Deoxyribonucleic Acid) polymerase, a fluorescence visual detection kit, a DNA template and ultrapure water, wherein the LAMP primer comprises outer primers F3 and B3 and inner primers FIP (Forward Inner Primer) and BIP (Backward Inner Primer). Specific detection and sensitive detection prove that an LAMP detection method provided by the invention can be used for monitoring a reaction in time, quantitatively detecting a copy number of the priB gene in the acute hepatopancreas necrosis syndrome and rapidly and accurately obtaining a detection result, thus bringing convenience for simply and rapidly detecting the acute hepatopancreas necrosis syndrome.

LAMP (Loop-Mediated Isothermal Amplification) kit for priA gene in acute hepatopancreas necrosis syndrome and application of LAMP kit

The invention discloses a method for efficiently shelling artemia salina eggs. The method comprises the steps that fresh water is used for soaking commodity artemia salina eggs, then soaking is conducted with seawater, and aeration is conducted sufficiently; then the eggs are sterilized and soaked in a sodium hypochlorite solution and aerated sufficiently, when the eggs become orange-red, eggs shells are separated out, the eggs are cleaned to remove residual chlorine and then put in an ethanol solution containing alkannin to be soaked for 40-60 minutes, and after soaking is finished, seawateris used for washing to remove residual ethanol from the artemia salina eggs. Accordingly, after the artemia salina eggs are shelled, keratin of an egg membrane can be further dissolved, the activity of embryos can be kept, the embryos of the eggs are fully exposed, and embryo development of the artemia salina eggs in the early stage can be observed directly under a microscope; in this way, the important guiding significance for family selection and breeding of artemia salina in the embryonic period is achieved, the artemia salina eggs are subjected to pathogen-free efficient and stable hatching, and then sufficient and high-quality bait is provided for fish and shrimp propagation and breeding; therefore, the method has the good promotion effect and application value for the aquatic breeding industry.

Method for efficiently shelling artemia salina eggs

The invention discloses a method and device for carrying out surface blowdown, blue-green alga treatment and oxygenation simultaneously. A drainage pipe is vertically arranged at the blowdown position in the center of a pond, a perforated blowdown pipe which is transversely connected to an elbow at the upper end of the drainage pipe and rotates around the center drainage pipe is used for carrying out surface blowdown and discharging blue-green algae at the same time, and a rotary aerator connected to the tail end of the blowdown pipe carries out oxygenation at the same time. Due to the fact that the drainage vertical pipe is fixed to the center of the pond, holes are formed in the transverse pipe, and one half of the drainage vertical pipe is immersed in surface-layer water, after the outer blowdown pipe is pulled out, only water on the surface flows in through the pipe holes; due to the fact that the transverse pipe rotates, all surface dirt is sucked into the pipe to be discharged. Due to the fact the blue-green algae generally exist on the surface layer of the water, the blue-green algae are sucked and discharged while the surface dirt is sucked and discharged, and the blue-green algae are treated. Due to the fact that the aerator is unfixed, the blue-green algae are started to push the pipe to rotate around the center pipe along the water surface, and the rotary aerator and the driven pipe can stir the surface water to achieve the aim of oxygenation.

Method and device for carrying out surface blowdown, blue-green alga treatment and oxygenation simultaneously

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Chen Xiaohan

Author Tools

Chat about Author