The invention relates to a plant polysaccharide extraction process, in particular to a response surface method optimized portulaca oleracea polysaccharide enzyme extraction process, and belongs to thetechnical field of food science and engineering. Based on single factor experiments, analysis is performed according to Design-Expert 8.0.6 software by a Box-Behnken response surface experiment design, a process for extracting polysaccharide from fresh portulaca oleracea juice by pectinase is optimized, an enzymolysis polysaccharide extraction regression model is built, enzyme extracted portulacaoleracea polysaccharide is obtained, the extraction process is simple, extraction conditions are mild, and the process is a proper method for extracting the portulaca oleracea polysaccharide.

Response surface method optimized portulaca oleracea polysaccharide enzyme extraction process

The present disclosure provides a treated product of purslane characterized by being obtained by treating purslane with hot water to obtain a hot water extract, and adding yeast to the hot water extract and culturing the resultant.

Purslane processed material, method for producing purslane processed material, supplement, medicine, intestinal mucosa protective agent, and intestinal regulator

The invention relates to a plant flavonoid extraction technology, in particular to a portulaca oleracea flavone enzyme extraction technology adopting an optimized response surface method, and belongsto the technical field of food science and engineering. The technology adopts Box-Behnken response surface experimental design on the basis of a single factor experiment, performs analysis according to Design-Expert 8.0.6 software, optimizes a flavone technology for extracting portulaca oleracea freshly squeezed juice through pectinase, establishes a regression model for extracting flavone throughan enzymolysis method, obtains enzyme extracted portulaca oleracea flavone enzyme, is simple in extraction technology and mild in extraction conditions, and belongs to a suitable method for extracting portulaca oleracea flavone.

Portulaca oleracea flavone enzyme extraction technology adopting optimized response surface method

The invention provides a method for extracting portulaca oleracea L. polysaccharide through pectinase. The crude Portulaca oleracea L. polysaccharide extracted through the method has good oxidation resistance. The method comprises the following steps that firstly, fresh portulaca oleracea L. is washed thoroughly, dried at the temperature of 50+/-1 DEG C, smashed, subjected to petroleum ether degreasing and dried at the temperature of 50+/-1 DEG C; secondly, ultrapure water and enzyme extraction is carried out, wherein the dry portulaca oleracea L. obtained in the first step is taken to be mixed with water, pH of a system is adjusted to be 4.8-5.2, the pectinase is added for extraction, enzymolysis is carried out at the temperature of 25-40 DEG C for 1-2 hours, and enzymatic hydrolysate is obtained; thirdly, the enzymatic hydrolysate is subjected to 100-DEG C boiling water bath to achieve enzyme inactivation, and after centrifugal separation is carried out, leach liquid containing the portulaca oleracea L. polysaccharide is obtained. The prepared portulaca oleracea L. polysaccharide is high in reducing capacity on Fe and Ce , high in clearance rate on hydroxide radical and superoxide anion free radical and good in oxidation resistance; compared with hot water extraction and ultrasonic-assisted extraction, the method has the advantage that the energy consumption is low. The antioxidant activity of the portulaca oleracea L. polysaccharide is superior to that of VC.

Method for extracting portulaca oleracea L. polysaccharide through pectinase

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Method for extracting portulaca oleracea L. polysaccharide through pectinase