The invention provides a compound microecological preparation as well as preparation and application thereof A preparation method of the compound microecological preparation comprises the steps of fermenting and culturing lactobacillus plantarum subspecies Zhang-LL and lactobacillus paracasei KL1 to obtain fermentation liquor, and separately centrifuging the fermentation liquor to obtain bacterial mud; separately mixing the two types of bacterial mud with a freeze-drying protective additive, then carrying out freeze-drying treatment, and compounding to obtain the compound microecological preparation The compound microecological preparation provided by the invention has a strong in-vitro salmonella inhibition effect, has an excellent preventive effect on salmonella pullorum disease, can inhibit the growth of salmonella enterica, maintains the intestinal micro-ecological balance of chicks, and has a good repair effect for the diseased tissues of the infected chicks; the compound microecological preparation can improve the growth performance of the chicks infected with the salmonella pullorum disease, improves the immunity of the chicks, and improves the anti-stress ability and antioxidant capacity of the organism; an effective method is provided for preventing the occurrence of chicken salmonellosis, and a thought is also provided for the development of a novel biological feedadditive

Compound microecological preparation as well as preparation and application thereof

The invention relates to a lactobacillus rhamnosus high-density bacterial solution and a preparation method of buried bacterial powder of the lactobacillus rhamnosus high-density bacterial solution. The lactobacillus rhamnosus high-density bacterial solution and the buried bacterial powder thereof are prepared by taking a yeast cell broken solution as an activator. The preparation method mainly comprises activating and fermenting. The preservation number of lactobacillus rhamnosus is CGMCC No.15557, and the preservation data is 8th, April, 2018. The lactobacillus rhamnosus high-density bacterial solution and the buried bacterial powder thereof prepared according to the method can be used for producing a fermenting agent for preparing a food additive, a feed additive or food and a feed taking functions of the lactobacillus rhamnosus as a core. The preparation method is simple, does not change the conventional fermenting steps, and is easy to operate and low in cost: the cost of saccharomycetes powder is about 3% lower than that of other hydrolyzing microbial nutrient preparation. The lactobacillus rhamnosus high-density bacterial solution is high in efficiency and high in nutrient utilization ratio and energy conversion proportion. The product is high in activity. The lactobacillus rhamnosus fermented by the method is applied to feed product and can play a role of replacing antibiotics partially.

Lactobacillus rhamnosus high-density bacterial solution and preparation method of buried bacterial powder of lactobacillus rhamnosus high-density bacterial solution

The invention relates to probiotic goat milk powder and a preparation method thereof. Medicinal and edible Chinese jujube polysaccharide is added to a culture medium to be used as a proliferation factor, a freezing-resistant factor and a freeze-drying protecting agent of probiotics, so that proliferation and freezing resistance of the probiotics are promoted; then at low temperature, cold treatment is performed, so that antifreeze protein produced through induction further improves the freezing resistance of the probiotics; and then through the composite freeze-drying protective agent, an icecrystal point is reduced, and protective effects on the probiotics are improved, so that high-activity probiotic powder is obtained. Desalinated whey powder and whey protein concentrate which are added to the probiotic goat milk powder are easy to digest and absorb; the added probiotics are high in viable count, so that the number of the probiotics in intestinal tracts of consumers can be increased, and microbial population can be regulated balanced; and dietary fiber glucan and xylooligosaccharide which are added can promote proliferation of the probiotics in the intestinal tracts, absorptionof minerals, promote squirm of the intestinal tracts and prevent constipation, and the probiotic goat milk powder can be beneficial to the health of the consumers.

Probiotic goat milk powder and preparation method thereof

The invention relates to probiotic goat milk tablets and a preparation method thereof. Medicinal and edible Chinese jujube polysaccharide is added to a culture medium to be used as a proliferation factor, a freezing-resistant factor and a freeze-drying protecting agent of probiotics, so that proliferation and freezing resistance of the probiotics are promoted; then at low temperature, cold treatment is performed, so that antifreeze protein produced through induction further improves the freezing resistance of the probiotics; and then through the composite freeze-drying protecting agent, an icecrystal point is reduced, and protecting effects on the probiotics are improved, so that high-activity probiotic powder is obtained. The probiotics added to the probiotic goat milk tablets are high in viable count, so that the number of the probiotics in intestinal tracts of consumers can be increased, and microbial population can be regulated to be balanced; and dietary fiber glucan and xylooligosaccharide which are added can promote proliferation of the probiotics in the intestinal tracts, absorption of minerals, promote squirm of the intestinal tracts and prevent constipation; and the added functional sweetening agent has low calorie, cannot cause decayed teeth or obesity, is free from influence on blood sugar, and has the functions of loosening bowel to relieve constipation, promotingcalcium absorption and the like.

Probiotic goat milk tablets and preparation method thereof

The invention relates to composite probiotic goat milk powder and a preparation method thereof. Medicinal and edible Chinese jujube polysaccharide is added to a culture medium to be used as a proliferation factor, a freezing-resistant factor and a freeze-drying protecting agent of probiotics, so that proliferation and freezing resistance of the probiotics are promoted; then at low temperature, cold treatment is performed, so that antifreeze protein produced through induction further improves the freezing resistance of the probiotics; and then through the composite freeze-drying protecting agent, an ice crystal point is reduced, and protecting effects on the probiotics are improved, so that high-activity probiotic powder is obtained. Desalinated whey powder and whey protein concentrate which are added to the probiotic goat milk powder are easy to digest and absorb; the added probiotics are high in viable count, so that the number of the probiotics in intestinal tracts of consumers can be increased, and microbial population can be regulated to be balanced; and dietary fiber glucan and xylooligosaccharide which are added can promote proliferation of the probiotics in the intestinal tracts, absorption of minerals, promote squirm of the intestinal tracts and prevent constipation, and the composite probiotic goat milk powder can be beneficial to health of the consumers.

Composite probiotic goat milk powder and preparation method thereof

The invention provides a micro-ecological preparation as well as a preparation method and application thereof. The micro-ecological preparation is prepared from single lactobacillus acidophilus bacterium powder and saccharomyces boulardii bacterium powder or a compound micro-ecological preparation of the two; the micro-ecological preparation is added into livestock and poultry feed for pigs, cattle, sheep and chickens according to the mass ratio of 0.05 percent to 0.2 percent and is uniformly mixed with the feed to prepare micro-ecological preparation feed. According to the micro-ecological preparation provided by the invention, lactobacillus acidophilus and saccharomyces boulardii can be used for improving intestinal flora imbalance of livestock and poultry and preventing diarrhea; inulinand galacto-oligosaccharides are added so that the absorption of the livestock and poultry on nutrient substances is promoted and the proliferation of intestinal probiotics is promoted; phytase is added so that the utilization rate on phytic acid in the feed of the livestock and poultry is increased, and the utilization rate of phosphorus salt and the emission of phosphorus are reduced; when themicro-ecological preparation provided by the invention is used, the disease-resisting capability of the livestock and poultry and the utilization capability on the feed are increased, and the growth speed is rapid.

Micro-ecological preparation as well as preparation method and application thereof

The present invention discloses a method of preparing goat milk active drink by using lactobacillus rhamnosus protease. A lactobacillus rhamnosus culture medium is inoculated with lactobacillus rhamnosus in an inoculation amount, culturing is carried out, the cultured lactobacillus rhamnosus is centrifuged, a centrifuged lactobacillus rhamnosus precipitate is collected, then the lactobacillus rhamnosus precipitate is washed using a sterile normal saline, and the washed lactobacillus rhamnosus is centrifuged to obtain lactobacillus rhamnosus paste; a phosphate buffer is added into the lactobacillus rhamnosus paste, the mixture is centrifuged to obtain a cell wall protease enzyme solution, and the cell wall protease enzyme solution is freeze-dried to obtain lactobacillus rhamnosus cell wallprotease bacterial powder; and the lactobacillus rhamnosus cell wall protease bacterial powder and CaCl2 are added into degreased fresh goat milk, enzymolysis is carried out, then beta-cyclodextrin, honey raisin tree fruit polysaccharides, erythritol, xylitol, L-malic acid, citric acid and sodium citrate are added, the materials are mixed evenly, the mixture is homogenized, and the homogenized mixture is sterilized to obtain the goat milk active drink containing antihypertensive peptides and anti-oxidation peptides. An ACE inhibitory activity is 70-90%, a DPPH free radical scavenging rate is 60-80% and a hydroxyl radical scavenging rate is 75-95%. The goat milk is used as a raw material, and the prepared goat milk active drink can be used as food for assisting lowering blood pressure, preventing oxidation, lowering blood sugar and preventing caries.

Method of preparing goat milk active drink by using lactobacillus rhamnosus protease

The present invention discloses a preparation method of sheep milk containing biological active peptides. Lactobacillus plantarum LP69 is inoculated into a MRS broth culture medium, after a culture, acentrifugation is conducted, a supernatant is discarded, a microorganism body precipitate is collected, and then the microorganism body precipitate is washed repeatedly with a Tris-HCL buffer for 3 times to obtain the microorganism body; the Tris-HCL buffer is added to the microorganism body to be stirred evenly, a centrifugation is conducted, and a resulting supernatant is cell wall proteases; skim sheep milk powder and distilled water are mixed to obtain skim sheep milk; and the skim sheep milk, cell wall proteases, concentrated fruit juice, beta-cyclodextrin, a compound stabilizer, white granulated sugar and citric acids are mixed evenly, and the mixture is homogenized to obtain the sheep milk containing the biological active peptides. The lactobacillus plantarum LP69 cell wall proteases are added into the sheep milk and can hydrolyze sheep milk proteins to produce ACE inhibitory peptides and antioxidant peptides. After drinking, the sheep milk has health-care functions of loweringblood pressure, preventing oxidation, etc. A long term drinking of the sheep milk has a pressure lowering function for populations with high blood pressure, is free of side effects, and can promote digestion and scavenge free radicals. An addition of fruit juice ingredients enables the sheep milk to be better in mouthfeel and the sheep milk helps digestion, beautifies features and nourished skin.

Preparation method of sheep milk containing biological active peptides

Disclosed are a culture medium to improve activity of Lactobacillus plantarum cell envelope proteinase and a preparation method thereof. The culture medium, per 1000 mL of aseptic solution, comprises10-30 g of glucose, 10-30 g of yeast extract powder, 0.02-0.05 g of manganese sulfate, 0.5-1.5 g of tween 80, 1-3 g of synanthrin, 3-5 g of casein peptone, 4-7 g of sodium hydrogen phosphate, 12-18 mgof leucine, and 10-20 ml of cucumber juice, and has initial pH value of 7.0-7.5. The culture medium provided herein has the advantages that the synanthrin, leucine and cucumber juice are added to a common basic medium so that good effect is provided for improving the activity of Lactobacillus plantarum cell envelope proteinase; these substances are low in price and easy to obtain, the preparationmethod is simple and easy, and large-scale production is benefited.

Culture medium to improve activity of Lactobacillus plantarum cell envelope proteinase and preparation method thereof

The invention relates to a method for preparing an antihypertensive-peptide-containing goat milk active beverage through Lactobacillus reuteri protease. The method comprises: adding a phosphoric acidbuffer solution to Lactobacillus reuteri mud, carrying out centrifugation to obtain a supernatant (the cell wall protease liquid), and carrying out freeze-drying to obtain Lactobacillus reuteri cell wall protease powder; and adding the actobacillus reuteri cell wall protease powder and CaCl2 to degreased fresh goat milk, carrying out enzymolysis, adding beta-cyclodextrin, the polysaccharide of Hovenia acerba Lindl, xylooligosaccharide, erythritol, xylitol, L-malic acid, citric acid and sodium citrate, uniformly mixing, homogenizing, and sterilizing to obtain the antihypertensive-peptide-containing goat milk active beverage. According to the present invention, the goat milk is used as the raw material, the GRAS microorganism Lactobacillus reuteri cell wall protease is used as the biocatalyst, the protein in the goat milk is hydrolyzed into the oligopeptide having ACE inhibition activity, and the prepared antihypertensive-peptide-containing goat milk active beverage can be used as the food for assisting blood pressure lowering, blood glucose lowering and decayed tooth prevention, and has multiple health care functions.

Method for preparing antihypertensive-peptide-containing goat milk active beverage through Lactobacillus reuteri protease

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