/pdf/induklenmis-pluripotent-kok-hucrelerin-elde-edilmesi-ve-2swvbuj8sq.pdf

İndüklenmiş Pluripotent Kök Hücrelerin Elde Edilmesi ve Rejeneratif Tıpta Uygulanabilirliği

[Background] We recently reported that human induced pluripotent stem (iPS) cells can be induced to differentiate into cardiomyocytes, and characterized their phenotype. Future investigation of hum...

Abstract 2296: Generation of Induced Pluripotent Stem Cells in Healthy Volunteers and Patients With Hereditary Heart Disease

OBJECTIVE
To learn the mechanisms between gut microbiome and the autoimmunity benefits on Traditional Chinese Medicine (TCM) in central nervous system (CNS), we investigated the neuro-protection effects and gut mircobiota changes of Heshouwu () on experimental autoimmune encepha-lomyelitis (EAE), an animal model of multiple sclerosis (MS).


METHODS
Mice were randomly divided into four groups: EAE mice (control phosphate-buffered saline group), 50 mg·kg·d Heshouwu ()-treated EAE mice, 100 mg·kg·d Heshouwu ()-treated EAE mice, and 200 mg·kg·d Heshouwu ()-treated EAE mice. The spinal cords were stained with hematoxylin and eosin (HE) and luxol fast blue for evaluating inflammatory infiltration and demyelination. The percentages of granulocyte macrophage-colony stimulating factor (GM-CSF)+CD4+, interleukin 17 (IL-17)+CD4+, Foxp3 CD4+, and interferon-γ (IFN-γ)+CD4+ T cells in the inguinal lymph nodes (LNs) and brain were determined by flow cytometry analysis. 16S rRNA gene sequencing was employed to analyze the changes in gut microbiota.


RESULTS
We found that Heshouwu () alleviated the disease severity and neuropathology of EAE as evaluated by clinical and histopathologyical scores. Heshouwu () increased the diversity and abundance of the gut microbiota, and decreased / ratio (F/B ratio). Heshouwu () also decreased the concentrations of IL-10, and IL-21 and increase the levels of GM-CSF, IL-17A, IL-17F and IL-22 in serum of EAE mice. Moreover, Heshouwu () modulated the T cell responses by inhibiting Th17 cells and restoring Treg cells in the small intestine lymphoid tissues and inguinal lymph nodes. Microbiota-depleted mice receiving Heshouwu ()-treated fecal microbiota trans-plantation had lower disease severity, neuropathology scores and alleviation of Th17/Treg imbalance compared to ad libitum group.


CONCLUSIONS
Our findings suggested that the vital neuro-protection role of Heshouwu () (TCM) in immunomodulation effects partly by regulations of gut microbiome.

Efficacy of Heshouwu () on gut mircobiota in mice with autoimmune encephalomyelitis.

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Abstract 2296: Generation of Induced Pluripotent Stem Cells in Healthy Volunteers and Patients With Hereditary Heart Disease

Efficacy of Heshouwu () on gut mircobiota in mice with autoimmune encephalomyelitis.