The invention provides, inter alia, methods for uniquely labeling populations of agents of interest using random combinations of oligonucleotides. The oligonucleotides may comprise a unique nucleotide sequence and/or one or more non-nucleic acid detectable moieties.

Compositions and methods for labeling of agents

The invention discloses a method for constructing a sequencing library and an application of the sequencing library. The method comprises the following steps: (a) connecting linkers with the two ends of double-stranded DNA fragments respectively so as to obtain linking products; (b) pyrolyzing the linking products into single-stranded DNA fragments; (c) screening the single-stranded DNA fragments by utilizing a probe; (d) carrying out chain extension reaction on the single-stranded DNA fragments by utilizing a first primer so as to obtain chain extension products; and (e) amplifying the chain extension products so as to obtain amplification products, wherein the amplification products form the sequencing library. The invention also discloses a sequencing method, a method for determining a nucleotide sequence, a device for constructing the sequencing library, sequencing equipment and a system for determining the nucleotide sequence.

Method for constructing sequencing library and application of sequencing library

The invention discloses a method and a device for gene sequencing of a plurality of mixed DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) sequences, which is mainly characterized by adding random labels at two ends of each DNA or RNA template sequence before establishing a bank. The method comprises the following steps of: mixing a first forward primer containing the random label and a connector, a first reverse primer and the DNA sequences, performing multiple PCR (Polymerase Chain Reaction) amplification on two PCR circulations, and purifying the PCR product to obtain a first DNA product; mixing the first DNA product, a second forward primer containing a sample indexing sequence and the connector, and a second reverse primer, performing PCR reaction to obtain a second PCR product; purifying to obtain a second DNA product; and sequencing the second DNA product to obtain a sequencing result of each DNA sequence. According to the method disclosed by the invention, by introducing the random labels to each DNA molecule, the sequencing precision is improved, the error rate of the sequencing is obviously reduced, and the copy number of each DNA is precisely detected.

Method and device for gene sequencing of plurality of mixed DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) sequences

The invention provides for methods for uniquely labeling populations of nucleic acids of interest in emulsion droplets using random combinations of oligonucleotides. The labeling methodology of the invention may be used, inter alia, to generate mate pair genomic fragments without the need for circularization. Because the method is independent of circularization, mate pairs can be generated from any length genomic fragment.

Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets

The invention provides an amplification sublibrary and a construction method thereof. The construction method comprises the following steps: S1, performing PCR amplification on target fragments of multiple samples to obtain a plurality of amplification products; S2, performing balanced mixing on the amplification products to obtain a mixed product; S3, sequentially performing fragmentation and terminal repairing on the mixed product, and adding A at a terminal 3' of the mixed product to obtain an A-repaired product; S4, jointing the A-repaired product by using a joint of PCR-free to obtain the amplification sublibrary. Through balanced mixing of the amplification products and through joint connection by using the optimized PCR-free, by the construction method, preference generated by PCR as well as chimeras in environmental samples is avoided, so that sequencing data between different samples have good consistency, subsequent clustering analysis and technical repeatability are more reasonable and more accurate, and can more really reflect evolutional relationship between the environmental samples.

Amplification sublibrary and construction method thereof

The invention designs 161 unique tag sequences with the lengths of 8 bp. Tags are embedded into a deoxyribonucleic acid (DNA) joint so as to form a DNA polymerase chain reaction (PCR)-free tag joint. The invention also provides a DNA tag library which introduces the tag sequence by connecting the DNA PCR-free tag joint without a PCR and is applied to solexa DNA sequencing. After a library construction method is optimized, the DNA tag library is constructed without the PCR.

Joint connection-based deoxyribonucleic acid (DNA) polymerase chain reaction (PCR)-free tag library construction method

DNA(deoxyribonucleic acid) index library building method based on PCR (polymerase chain reaction)

DNA index library building method based on high throughput sequencing

Tag library constructing method based on DNA (deoxyribonucleic acid) adapter connection as well as used tag and tag adapter

Provided are DNA tags for constructing a DNA tag library, oligonucleotides, DNA tag libraries and a method for preparing the same, a method for determining the sequence information of a DNA sample, a method for determining the sequence information of a plurality of DNA samples, and a kit for constructing the DNA tag libraries. Said DNA tags consist of the nucleotides shown in SEQ ID NO: (2N-1), in which N is any integer from 1 to 161.

Dna tags and use thereof

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