Charles A. Smith
Allegheny General Hospital
13 Papers
96 Citations
Charles A. Smith is an academic researcher from Allegheny General Hospital. The author has contributed to research in topics: Cancer & Laser Scanning Cytometry. The author has an hindex of 9, co-authored 13 publications.
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Papers
Sequential paraformaldehyde and methanol fixation for simultaneous flow cytometric analysis of DNA, cell surface proteins, and intracellular proteins.
Agnese A. Pollice,McCoy Jp,Stanley E. Shackney,Charles A. Smith,Agarwal J,D. R. Burholt,Laura E. Janocko,Francis J. Hornicek,Sarita G. Singh,Robert J. Hartsock +9 more
TL;DR: A cell fixation and permeabilization procedure consisting of sequential paraformaldehyde and methanol was evaluated and found suitable for concomitant flow cytometric quantification of total cellular DNA, immunofluorescence measurements of cell surface proteins, and immunof LU measurements of intracellular proteins.
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Origins and clinical implications of aneuploidy in early bladder cancer.
Stanley E. Shackney,George Berg,Sheryl R. Simon,Jeffrey Cohen,Suresh Amina,William Pommersheim,Robert Yakulis,Scott Wang,Michelle Uhl,Charles A. Smith,Agnese A. Pollice,Robert J. Hartsock +11 more
TL;DR: It is concluded that DNA multiploidy and hypertetraploidy are markers of advanced stages of genetic evolution in human bladder cancer.
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Discrepancies between flow cytometric and cytogenetic studies in the detection of aneuploidy in human solid tumors
Stanley E. Shackney,D. R. Burholt,Agnese A. Pollice,Charles A. Smith,Reginald P. Pugh,Robert J. Hartsock +5 more
TL;DR: Overall, 13/20 or 65% of patients with solid tumors in this study had numerical chromosomal abnormalities that were not detected by flow cytometry, and among the tumor cell lines established in culture, the DNA Index was often higher than the cytogenetic index.
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Distinctive patterns of Her-2/neu, c-myc, and cyclin D1 gene amplification by fluorescence in situ hybridization in primary human breast cancers.
Laura E. Janocko,Kathryn A. Brown,Charles A. Smith,Ling Ping Gu,Agnese A. Pollice,Sarita G. Singh,Thomas B. Julian,Norman Wolmark,Lillian Sweeney,Jan F. Silverman,Stanley E. Shackney +10 more
TL;DR: The data suggest that in tumors containing multiple abnormalities, these abnormalities often accumulate in the same cells within each tumor, and the same patterns of accumulation of multiple genophenotypic abnormalities are recapitulated in different tumors.
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•Journal Article
The accumulation of multiple genetic abnormalities in individual tumor cells in human breast cancers: clinical prognostic implications.
S. E. Shackney,Agnese A. Pollice,Charles A. Smith,L Alston,Sarita G. Singh,Laura E. Janocko,K A Brown,S Petruolo,D W Groft,R Yakulis,Robert J. Hartsock +10 more
TL;DR: The number of genetic abnormalities that accumulate in individual cells in primary breast cancers reflects the degree of advancement of a tumor in its genetic evolutionary sequence, and provides useful clinical prognostic information.
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