Catherine A. Freije
Harvard University
35 Papers
904 Citations
Catherine A. Freije is an academic researcher from Harvard University. The author has contributed to research in topics: CRISPR & Medicine. The author has an hindex of 17, co-authored 30 publications. Previous affiliations of Catherine A. Freije include Rockefeller University & Broad Institute.
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Papers
Nucleic acid detection with CRISPR-Cas13a/C2c2
Jonathan S. Gootenberg,Omar O. Abudayyeh,Jeong Wook Lee,Patrick Essletzbichler,Aaron J. Dy,Aaron J. Dy,Julia Joung,Vanessa Verdine,Nina M. Donghia,Nichole M. Daringer,Catherine A. Freije,Catherine A. Freije,Cameron Myhrvold,Cameron Myhrvold,Roby P. Bhattacharyya,Jonathan Livny,Aviv Regev,Aviv Regev,Eugene V. Koonin,Deborah T. Hung,Pardis C. Sabeti,James J. Collins,Feng Zhang +22 more
TL;DR: A Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), is used to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA.
Field-deployable viral diagnostics using CRISPR-Cas13
Cameron Myhrvold,Cameron Myhrvold,Catherine A. Freije,Catherine A. Freije,Jonathan S. Gootenberg,Omar O. Abudayyeh,Hayden C. Metsky,Ann Durbin,Ann Durbin,Max J. Kellner,Amanda L Tan,Lauren M. Paul,Leda Parham,Kimberly García,Kayla G. Barnes,Kayla G. Barnes,Bridget Chak,Bridget Chak,Adriano Mondini,Maurício Lacerda Nogueira,Sharon Isern,Scott F. Michael,Ivette Lorenzana,Nathan L. Yozwiak,Nathan L. Yozwiak,Bronwyn MacInnis,Bronwyn MacInnis,Irene Bosch,Irene Bosch,Lee Gehrke,Lee Gehrke,Feng Zhang,Pardis C. Sabeti +32 more
TL;DR: The Cas13-based SHERLOCK platform can detect Zika virus and dengue virus in patient samples at concentrations as low as 1 copy per microliter and can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015–2016 pandemic.
1.1K
Nucleic acid detection with CRISPR-Cas13a/C2c2
Jonathan S. Gootenberg,Omar O. Abudayyeh,Jeong Wook Lee,Patrick Essletzbichler,Aaron J. Dy,Aaron J. Dy,Julia Joung,Vanessa Verdine,Nina M. Donghia,Nichole M. Daringer,Catherine A. Freije,Catherine A. Freije,Cameron Myhrvold,Cameron Myhrvold,Roby P. Bhattacharyya,Jonathan Livny,Aviv Regev,Aviv Regev,Eugene V. Koonin,Deborah T. Hung,Pardis C. Sabeti,James J. Collins,Feng Zhang +22 more
- 01 Apr 2017
TL;DR: In this paper, a CRISPR-Dx-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter Unlocking (SHERLOCK), was proposed to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA.
740
Massively multiplexed nucleic acid detection with Cas13.
Cheri M. Ackerman,Cameron Myhrvold,Cameron Myhrvold,Sri Gowtham Thakku,Sri Gowtham Thakku,Catherine A. Freije,Catherine A. Freije,Hayden C. Metsky,David Yang,Simon Ye,Simon Ye,Chloe K. Boehm,Tinna Solveig F. Kosoko-Thoroddsen,Jared Kehe,Tien G. Nguyen,Amber Carter,Anthony Kulesa,John R. Barnes,Vivien G. Dugan,Deborah T. Hung,Deborah T. Hung,Paul C. Blainey,Pardis C. Sabeti +22 more
TL;DR: CRISPR-based nucleic acid detection is used in a platform that can simultaneously detect 169 human-associated viruses in multiple samples, providing scalable, multiplexed pathogen detection aimed at routine surveillance for public health.
Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
Jon Arizti-Sanz,Catherine A. Freije,Catherine A. Freije,Alexandra C. Stanton,Alexandra C. Stanton,Brittany A. Petros,Chloe K. Boehm,Sameed Siddiqui,Bennett M. Shaw,Bennett M. Shaw,Gordon Adams,Tinna Solveig F. Kosoko-Thoroddsen,Molly Kemball,Jessica N. Uwanibe,Jessica N. Uwanibe,Fehintola V. Ajogbasile,Fehintola V. Ajogbasile,Philomena Eromon,Robin Gross,Loni Wronka,Katie Caviness,Lisa E. Hensley,Nicholas H. Bergman,Bronwyn MacInnis,Bronwyn MacInnis,Christian T. Happi,Christian T. Happi,Christian T. Happi,Jacob E. Lemieux,Jacob E. Lemieux,Pardis C. Sabeti,Cameron Myhrvold +31 more
TL;DR: SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples is developed, demonstrating 90% sensitivity and 100% specificity.