Candice E. Coombes
Johns Hopkins University School of Medicine
9 Papers
24 Citations
Candice E. Coombes is an academic researcher from Johns Hopkins University School of Medicine. The author has contributed to research in topics: Gene & Retrotransposon. The author has an hindex of 8, co-authored 8 publications. Previous affiliations of Candice E. Coombes include Johns Hopkins University.
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Papers
Synthetic chromosome arms function in yeast and generate phenotypic diversity by design
Jessica S. Dymond,Sarah M. Richardson,Candice E. Coombes,Timothy D. Babatz,Héloïse Muller,Narayana Annaluru,William Jeremy Blake,Joy Wu Schwerzmann,Joy Wu Schwerzmann,Junbiao Dai,Junbiao Dai,Derek L. Lindstrom,Derek L. Lindstrom,Annabel C. Boeke,Annabel C. Boeke,Daniel E. Gottschling,Srinivasan Chandrasegaran,Joel S. Bader,Jef D. Boeke +18 more
TL;DR: This work describes a synthetic yeast genome project, Sc2.0, and the first partially synthetic eukaryotic chromosomes, Saccharomyces cerevisiae chromosome synIXR, and semi-synVIL, and shows the utility of SCRaMbLE as a novel method of combinatorial mutagenesis, capable of generating complex genotypes and a broad variety of phenotypes.
Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae
Shay Ben-Aroya,Candice E. Coombes,Teresa Kwok,Kathryn A. O'Donnell,Jef D. Boeke,Philip Hieter +5 more
TL;DR: A systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general is developed.
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GTPase activity plays a key role in the pathobiology of LRRK2
Yulan Xiong,Candice E. Coombes,Austin S. Kilaru,Xiaojie Li,Aaron D. Gitler,William J. Bowers,Valina L. Dawson,Ted M. Dawson,Darren J. Moore,Darren J. Moore +9 more
TL;DR: A model of LRRK2 cytotoxicity in the baker's yeast Saccharomyces cerevisiae is developed and it is suggested that the GTPase domain may contribute to the toxicity of L RRK2.
An evaluation of detection methods for large lariat RNAs
Candice E. Coombes,Jef D. Boeke +1 more
TL;DR: Using a wide range of techniques, it is demonstrated that some of the techniques used in the initial study describing the lariat are capable of incorrectly reporting a lariat structure, and the role of the Dbr1 protein in Ty1 retrotransposition remains elusive.
Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon
TL;DR: A modular mini-Ty1 element encoding the minimal sequence that can be retrotransposed by the Ty1 proteins, supplied in trans by a helper construct is described and suggests that the intramolecular interaction between the 5'-GAGGAGA and UCUCCUC sequences stabilizes an RNA structure required for efficient initiation of reverse transcription.