C.E. Kuster
University of Illinois at Urbana–Champaign
11 Papers
117 Citations
C.E. Kuster is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Semen & Sperm. The author has an hindex of 8, co-authored 10 publications.
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Papers
Field investigations of bacterial contaminants and their effects on extended porcine semen.
TL;DR: A minimum contamination technique (MCT) protocol was developed to standardize hygiene and sanitation during boar preparation, semen collection, semen processing and laboratory sanitation, which resulted in the control of bacterial contamination in the extended semen.
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Capillary-loaded particle fluid dynamics: effect on estimation of sperm concentration
TL;DR: Assessment of chamber flow dynamics, flow uniformity, inflow cell velocity, and results of concentration measurements under different flow conditions for latex bead and porcine and human sperm suspensions provided further support that hemacytometry, when performed properly, remains the gold standard.
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Particle distribution in low-volume capillary-loaded chambers.
TL;DR: Differences between CASA results and hemacytometry are established, in large part, due to the Segre-Silberberg effect, which occurs during Poiseuille flow in high-gradient fluid flow in thin capillary-loaded slides.
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Determining sample size for the morphological assessment of sperm.
TL;DR: Application of basic statistical principles such as the nature of proportions, level of confidence in an observed value, and the interaction of sample size with precision can and should be used in the decision process regarding the number of spermatozoa to include in the morphology assessment.
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Immunofluorescence reveals ubiquitination of retained distal cytoplasmic droplets on ejaculated porcine spermatozoa.
TL;DR: The presence of antigens recognized by anti-ubiquitin antibodies in the boar sperm CD, coupled with the possibility that superfluous ubiquitin species are detrimental to embryonic development by targeting critical paternally contributed zygotic organelles, raises concerns that retained DDs may be more detrimental to fertility than previously suspected.
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