Brian Ward
Syracuse University
17 Papers
454 Citations
Brian Ward is an academic researcher from Syracuse University. The author has contributed to research in topics: Footprinting & DNA. The author has an hindex of 15, co-authored 17 publications.
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Papers
Molecular recognition between oligopeptides and nucleic acids: novel imidazole-containing oligopeptides related to netropsin that exhibit altered DNA sequence specificity.
TL;DR: Oligopeptides have been synthesized that are structurally related to the antiviral antitumor antibiotic netropsin, but in which each of the pyrrole units is successively replaced by an imidazole moiety, as well as their di- and triimidazoles-containing counterparts.
204
Esperamicins, a class of potent antitumor antibiotics: mechanism of action.
Byron H. Long,Jerzy Golik,Salvatore Forenza,Brian Ward,Robert Rehfuss,James C. Dabrowiak,Joseph J. Catino,Steve T. Musial,Kenneth W. Brookshire,Terrence W. Doyle +9 more
TL;DR: Results suggest that the pendant aromatic chromophore of esperamicin A1 may contribute to the uptake of the drug into cells but may also hinder double-strand DNA break formation, and it is likely that this diradical is the active form of this class of antitumor agents.
116
DNA cleavage specificity of a group of cationic metalloporphyrins.
TL;DR: Analysis of the mechanism of porphyrin-mediated strand breakage in terms of the DNA cleavage mechanism of methidium-propyl-iron-EDTA and Fe-bleomycin, the potential of the cationic metalloporphyrins as footprinting probes and as new "reporter ligands" for DNA is presented and discussed.
109
DNA binding specificity of a series of cationic metalloporphyrin complexes.
TL;DR: The sequence specificities of a series of cationic metalloporphyrins toward a 139 base pair restriction fragment of pBR-322 DNA have been studied by DNase I footprinting methodology and it was revealed that the 5- and 6-coordinate complexes of meso-tetrakis(N-methyl-4-pyridiniumyl)porphine, MT4MPyP, were found to bind to AT regions of DNA.
97
Determination of netropsin-DNA binding constants from footprinting data.
TL;DR: A theory for deriving drug-DNA site binding constants from footprinting data and sites containing the dinucleotide sequence 5'-TA-3' were found to have significantly lower binding constants than those without this sequence, suggesting that an adenine-adenine clash produces a DNA structural alteration in the minor groove which discourages netropsin binding to DNA.