Brian Sit
University of Hong Kong
6 Papers
2 Citations
Brian Sit is an academic researcher from University of Hong Kong. The author has contributed to research in topics: Podosome & Vascular smooth muscle. The author has an hindex of 2, co-authored 4 publications. Previous affiliations of Brian Sit include King's College London & Randall Division of Cell and Molecular Biophysics.
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Papers
Costameres, dense plaques and podosomes: the cell matrix adhesions in cardiovascular mechanosensing.
TL;DR: The evidence for mechanical sensing in the cardiovascular system is looked at, before comparing the different cytoskeletal arrangements and adhesion sites in cardiomyocytes and vascular smooth muscle cells and what is known about mechanical sensing through the various structures.
Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching
Pamela Swiatlowska,Brian Sit,Zheng Feng,Emilie Marhuenda,Ioannis Xanthis,Simona Zingaro,Matthew Ward,Xinmiao Zhou,Qingzhong Xiao,Catherine M. Shanahan,Gareth E. Jones,Cheng-han Yu,Thomas Iskratsch +12 more
TL;DR: It is found that pressure and stiffness individually affect the VSMC phenotype, but only the combination of hypertensive pressure and matrix compliance, and as such mechanical stimuli that are prevalent during atherosclerosis, leads to a full phenotypic switch including the formation of matrix-degrading podosomes.
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Tail domains of myosin-1e regulate phosphatidylinositol signaling and F-actin polymerization at the ventral layer of podosomes
TL;DR: It is reported that long-tailed myosin-1e (Myo1e) is enriched at the ventral layer of the podosome core in a PI(3,4,5)P3-dependent manner and serves as a regulatory component to connect phosphatidylinositol signaling to F-actin polymerization at the podsome.
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Matrix stiffness and blood pressure together regulate vascular smooth muscle cell phenotype switching and cofilin dependent podosome formation
Brian Sit,Brian Sit,Brian Sit,Zhen Feng,Ioannis Xanthis,Emilie Marhuenda,Simona Zingaro,Catherine M. Shanahan,Gareth E. Jones,Cheng-han Yu,Thomas Iskratsch,Thomas Iskratsch +11 more
TL;DR: In this paper, the impact of mechanical and chemical stimulations on vascular smooth muscle cells (VSMCs) phenotypic switching was systematically studied and cofilin was identified as a key modulator of mechanosensitive phenotype switch, which is regulated through Ca2+/slingshot-dependent pressure sensing and RhoA/ROCK-dependent stiffness sensing pathways.