Brian A. Learn
Johns Hopkins University
4 Papers
180 Citations
Brian A. Learn is an academic researcher from Johns Hopkins University. The author has contributed to research in topics: CRISPR & DnaA. The author has an hindex of 4, co-authored 4 publications.
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Papers
Recognition, Targeting, and Hydrolysis of the λ O Replication Protein by the ClpP/ClpX Protease
Malgorzata Gonciarz-Swiatek,Alicja Wawrzynów,Soo Jong Um,Brian A. Learn,Roger McMacken,William L. Kelley,Costa Georgopoulos,Olaf Sliekers,Maciej Zylicz +8 more
TL;DR: It is suggested that two distinct structural elements may be required in substrate polypeptides to enable efficient hydrolysis by the ClpP/ClpX protease: (i) a ClpX-binding site, which may be located remotely from substrate termini, and (ii) a proper N- or C-terminal sequence, whose exposure on the substrate surface may be induced by the binding of Clp X.
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Cryptic single-stranded-DNA binding activities of the phage λ P and Escherichia coli DnaC replication initiation proteins facilitate the transfer of E. coli DnaB helicase onto DNA
TL;DR: This work investigates the interaction of replication initiation proteins with single-stranded DNA (ssDNA) and finds that the lambda O replication initiator enhances interaction of the P x DnaB complex with ssDNA.
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Processing and integration of functionally oriented prespacers in the Escherichia coli CRISPR system depends on bacterial host exonucleases
TL;DR: It is shown that the bacterial 3′–5′ exonucleases DnaQ and ExoT can trim long 3′ overhangs of prespacers and promote integration in the correct orientation, and found that trimming by these exon nucleases results in an asymmetric intermediate, because Cas1-Cas2 protects the PAM sequence, which helps to define spacer orientation.
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Target sequence requirements of a type III-B CRISPR-Cas immune system.
TL;DR: The target requirement for the type III-B system from T. maritima is defined and a framework for understanding the target requirements of type III systems as a whole is provided.
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