Bradley M. Downs
Johns Hopkins University School of Medicine
15 Papers
8 Citations
Bradley M. Downs is an academic researcher from Johns Hopkins University School of Medicine. The author has contributed to research in topics: Medicine & DNA methylation. The author has an hindex of 3, co-authored 8 publications. Previous affiliations of Bradley M. Downs include Johns Hopkins University.
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Papers
Targeted nanopore sequencing with Cas9-guided adapter ligation
Timothy Gilpatrick,Isac Lee,James E. Graham,Etienne Raimondeau,Rebecca Bowen,Andrew John Heron,Bradley M. Downs,Saraswati Sukumar,Fritz J. Sedlazeck,Winston Timp +9 more
TL;DR: N nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy that uses targeted cleavage of chromosomal DNA with Cas9 to ligate adapters for nanopore sequencing, is described and shown that nCATS can simultaneously assess haplotype-resolved single-nucleotide variants, structural variations and CpG methylation.
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DNA Methylation Markers for Breast Cancer Detection in the Developing World.
Bradley M. Downs,Claudia Mercado-Rodriguez,Ashley Cimino-Mathews,Chuang Chen,Jing Ping Yuan,Eunice van den Berg,Leslie Cope,Fernando Schmitt,Gary Tse,Syed Z. Ali,Danielle Meir-Levi,Rupali Sood,Juanjuan Li,Juanjuan Li,Andrea L. Richardson,Marina Mosunjac,Monica Rizzo,Suzana Tulac,Kriszten Kocmond,Timothy de Guzman,Edwin W. Lai,Brian Rhees,Michael Bates,Antonio C. Wolff,Edward Gabrielson,Susan C. Harvey,Christopher B. Umbricht,Kala Visvanathan,Kala Visvanathan,Mary Jo Fackler,Saraswati Sukumar +30 more
TL;DR: A fast and accurate methylation marker–based automated cartridge system to detect breast cancer in FNA samples has the potential to prioritize cancer over benign tissues for expedited pathologic evaluation in poorly resourced countries.
Methylated markers accurately distinguish primary central nervous system lymphomas (PCNSL) from other CNS tumors.
Bradley M. Downs,Wanjun Ding,Leslie Cope,Christopher B. Umbricht,Wenge Li,Huihua He,Xiaokang Ke,Matthias Holdhoff,Chetan Bettegowda,Weiping Tao,Saraswati Sukumar +10 more
TL;DR: In this paper, the authors identified and validated tumor-specific markers that can distinguish primary central nervous system lymphoma (PCNSL) from other primary CNS tumors in tissues, and developed a novel, efficient assay called Tailed Amplicon Multiplexed-Methylation-Specific PCR (TAM-MSP).
The biological function of tumor-derived extracellular vesicles on metabolism
TL;DR: In this paper , the authors summarize the finding of studies focused on measuring the effects of TDE on tumor-related microenvironment and systemic metabolism, and apply the key bioactive cargoes of exosomes to provide new strategies for the treatment of tumors.
Automated and rapid detection of cancer in suspicious axillary lymph nodes in patients with breast cancer.
Juanjuan Li,Juanjuan Li,Bradley M. Downs,Leslie Cope,Mary Jo Fackler,Xiuyun Zhang,Chuan gui Song,Christopher J. VandenBussche,Kejing Zhang,Yong Han,Yufei Liu,Suzana Tulac,Neesha Venkatesan,Timothy de Guzman,Chuang Chen,Edwin W. Lai,Jingping Yuan,Saraswati Sukumar +17 more
- 07 Jul 2021
TL;DR: In this paper, the authors proposed a 5-h automated GeneXpert system-based prototype breast cancer detection assay (BCDA) that quantitatively measures DNA methylation in ten tumor-specific gene markers.