Bolin Wang
University of Leeds
8 Papers
Bolin Wang is an academic researcher from University of Leeds. The author has contributed to research in topics: Chemistry & Membrane protein. The author has an hindex of 1, co-authored 4 publications. Previous affiliations of Bolin Wang include University of St Andrews.
Chat about Author
Papers
Allosteric activation of an ion channel triggered by modification of mechanosensitive nano-pockets.
Charalampos Kapsalis,Bolin Wang,Bolin Wang,Hassane El Mkami,Samantha J. Pitt,Jason R. Schnell,Terry K. Smith,Jonathan D. Lippiat,Bela E. Bode,Christos Pliotas +9 more
TL;DR: PELDOR/DEER spectroscopy is used to identify a single site which generated an allosteric structural response in the absence of membrane tension, and restricts lipid access to the nano-pocket, interrupting the contact between the membrane and the channel that mediates mechanosensitivity.
Using pulsed EPR in the structural analysis of integral membrane proteins
Andrew M. Hartley,Yue Ma,Benjamin J. Lane,Bolin Wang,Christos Pliotas +4 more
- 16 Nov 2020
TL;DR: PELDOR distance measurements can provide quantitative information on the conformational equilibria of membrane proteins, dovetailing well with techniques such as X-ray crystallography and cryo-EM as discussed by the authors.
10
Tension mediated mechanical activation and pocket delipidation lead to an analogous MscL state
Bolin Wang,Benjamin J. Lane,Charalampos Kapsalis,James R. Ault,Frank Sobott,Hassane El Mkami,Antonio N. Calabrese,Antreas C. Kalli,Christos Pliotas +8 more
TL;DR: In this article, the authors combined pulsed-EPR and HDX-MS measurements on MscL, coupled with molecular dynamics under membrane tension, to investigate the changes associated with the distinctively derived states.
Mechanical and molecular activation lead to structurally analogous MscL states
Bolin Wang,Benjamin J. Lane,Charalampos Kapsalis,James R. Ault,Frank Sobott,Hassane El Mkami,Antonio N. Calabrese,Antreas C. Kalli,Christos Pliotas,Christos Pliotas +9 more
TL;DR: Experimental data: raw data for 3pESEEM time-domain traces Computational data: Raw molecular dynamic simulation trajectories as discussed by the authors, which is used for 3PESEEM simulations.