Bo Chen
Columbia University
17 Papers
15 Citations
Bo Chen is an academic researcher from Columbia University. The author has contributed to research in topics: Biology & Ribosome. The author has an hindex of 9, co-authored 13 publications.
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Papers
The ABC-F protein EttA gates ribosome entry into the translation elongation cycle.
Grégory Boël,Paul Smith,Wei Ning,Michael T. Englander,Bo Chen,Yaser Hashem,Anthony J Testa,Jeffrey J. Fischer,Hans-Joachim Wieden,Joachim Frank,Ruben L. Gonzalez,John F. Hunt,John F. Hunt +12 more
TL;DR: It is demonstrated that YjjK, the most prevalent eubacterial ABC-F protein, gates ribosome entry into the translation elongation cycle through a nucleotide-dependent interaction sensitive to ATP/ADP ratio.
Late steps in bacterial translation initiation visualized using time-resolved cryo-EM.
Sandip Kaledhonkar,Ziao Fu,Kelvin Caban,Wen Li,Bo Chen,Ming Sun,Ruben L. Gonzalez,Joachim Frank +7 more
TL;DR: The power of time-resolved cryogenic electron microscopy is demonstrated to determine how a time-ordered series of conformational changes contribute to the mechanism and regulation of one of the most fundamental processes in biology.
A Fast and Effective Microfluidic Spraying-Plunging Method for High-Resolution Single-Particle Cryo-EM.
Xiangsong Feng,Ziao Fu,Sandip Kaledhonkar,Yuan Jia,Binita Shah,Amy Jin,Zheng Liu,Ming Sun,Bo Chen,Robert A. Grassucci,Yukun Ren,Hongyuan Jiang,Joachim Frank,Joachim Frank,Qiao Lin +14 more
TL;DR: A spraying-plunging method for preparing cryoelectron microscopy grids with vitreous ice of controllable, highly consistent thickness using a microfluidic device is described and it is demonstrated that the structure can be solved to high resolution with this method of sample preparation.
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Structural dynamics of ribosome subunit association studied by mixing-spraying time-resolved cryogenic electron microscopy.
Bo Chen,Sandip Kaledhonkar,Ming Sun,Bingxin Shen,Zonghuan Lu,David Barnard,Toh-Ming Lu,Ruben L. Gonzalez,Joachim Frank,Joachim Frank +9 more
TL;DR: This work used a recently developed mixing-spraying, time-resolved, cryogenic electron microscopy (cryo-EM) method to study ribosomal subunit association in the sub-second time range and identified three distinct ribosome conformations in the associated ribosomes.
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Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy
Ziao Fu,Sandip Kaledhonkar,Anneli Borg,Ming Sun,Bo Chen,Robert A. Grassucci,Robert A. Grassucci,Måns Ehrenberg,Joachim Frank,Joachim Frank +9 more
TL;DR: A mixing-spraying-based method of time-resolved cryo-electron microscopy (cryo-EM) is used to visualize the short-lived intermediates of the recycling process, and the two complexes that contain both RRF and EF-G bound to the PostTC or deacylated tRNA bound toThe 30S subunit are of particular interest.
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