Birgit Schäfer
Goethe University Frankfurt
19 Papers
1 Citations
Birgit Schäfer is an academic researcher from Goethe University Frankfurt. The author has contributed to research in topics: Transactivation & Chemistry. The author has an hindex of 15, co-authored 18 publications.
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Papers
Conformational Switches Modulate Protein Interactions in Peptide Antibiotic Synthetases
Alexander Koglin,Mohammad Reza Mofid,Frank Löhr,Birgit Schäfer,Vladimir V. Rogov,Marc-Michael Blum,Tanja Mittag,Mohamed A. Marahiel,Frank Bernhard,Volker Dötsch +9 more
TL;DR: This work describes the different states of a peptidyl carrier protein (PCP) that play a crucial role in its function as a peptide shuttle in the nonribosomal peptide synthetases of the tyrocidine A system.
163
Oocyte DNA damage quality control requires consecutive interplay of CHK2 and CK1 to activate p63
Marcel Tuppi,Sebastian Kehrloesser,D. Coutandin,Valerio Rossi,Laura M. Luh,Alexander Strubel,Katharina Hötte,Meike Hoffmeister,Birgit Schäfer,Tiago De Oliveira,Florian R. Greten,Ernst H. K. Stelzer,Stefan Knapp,Stefan Knapp,Massimo De Felici,Christian Behrends,Francesca Gioia Klinger,Volker Dötsch +17 more
TL;DR: The first detailed mechanistic insight is provided into the activation of p63, a process that requires phosphorylation by both the priming kinase CHK2 and the executioner kinase CK1 in mouse primordial follicles, thus uncovering a new target for the development of fertoprotective therapies.
148
Conformational stability and activity of p73 require a second helix in the tetramerization domain.
D. Coutandin,Frank Löhr,Frank H. Niesen,Teppei Ikeya,Tobias Alexander Weber,Birgit Schäfer,E M Zielonka,E M Zielonka,Alex N. Bullock,Annie Yang,Peter Güntert,Peter Güntert,Stefan Knapp,Frank McKeon,Frank McKeon,Horng Der Ou,Horng Der Ou,Volker Dötsch +17 more
TL;DR: Analysis of the ODs of p73 and p63 shows that the heterotetramer consisting of two homodimers is thermodynamically more stable than the two homotetramers, supporting the notion of functional orthogonality within the p53 family.
Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: a case study of the human endothelin A receptor.
Friederike Junge,Laura M. Luh,Davide Proverbio,Birgit Schäfer,Rupert Abele,Michael Beyermann,Volker Dötsch,Frank Bernhard +7 more
TL;DR: This is a first quality evaluation of a membrane protein obtained in two different cell-free expression modes and it is demonstrated that both can be used for the production of ligand-binding competent endothelin A receptor in quantities sufficient for structural approaches.
46
Analysis of the oligomeric state and transactivation potential of TAp73α.
Laura M. Luh,Sebastian Kehrloesser,Gregor B. Deutsch,Jakob Gebel,D. Coutandin,Birgit Schäfer,Massimiliano Agostini,Gerry Melino,Gerry Melino,Gerry Melino,Volker Dötsch +10 more
TL;DR: The oligomeric state of TAp73α, the isoform responsible for the quality control in oocytes, is investigated by size exclusion chromatography and detailed domain interaction mapping, and it is implied that the regulation of p73′s transcriptional activity might be more closely related to p53 than to p63.