Bernardo Abecasis
Universidade Nova de Lisboa
8 Papers
10 Citations
Bernardo Abecasis is an academic researcher from Universidade Nova de Lisboa. The author has contributed to research in topics: Induced pluripotent stem cell & Chemistry. The author has an hindex of 4, co-authored 8 publications. Previous affiliations of Bernardo Abecasis include University of Lisbon.
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Papers
Expansion of 3D human induced pluripotent stem cell aggregates in bioreactors: Bioprocess intensification and scaling-up approaches
Bernardo Abecasis,Tiago Aguiar,Emilie Arnault,Rita Costa,Patrícia Gomes-Alves,Anders Aspegren,Margarida Serra,Paula M. Alves +7 more
TL;DR: Environmental controlled stirred-tank bioreactors operating in perfusion are used as a powerful tool for bioprocess intensification of hiPSC production and demonstrate the importance of controlling the dissolved oxygen concentration at low levels and perfusion at 1.3day-1 dilution rate to improveHiPSC growth as aggregates in a xeno-free medium.
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Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes
P. José Gouveia,S.C. Rosa,Leonardo Ricotti,Bernardo Abecasis,Henrique V. Almeida,L. R. Monteiro,J. B. Nunes,F. Sofia Carvalho,Margarida Serra,Sergey Yu. Luchkin,A. Leonidovitch Kholkin,A. Leonidovitch Kholkin,P. Marques Alves,P. Jorge Oliveira,Rose Cristina Veiga Gomes de Carvalho,Arianna Menciassi,R. Pires das Neves,L. Silva Ferreira +17 more
TL;DR: The results indicate that MNF+PIEZO scaffold promotes rat and human cardiac cell attachment and alignment, maintains the ratio of cell populations overtime, promotes cell-cell communication and metabolic maturation, and preserves cardiomyocyte (CM) contractility for at least 12 days.
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Human Extracellular-Matrix Functionalization of 3D hiPSC-Based Cardiac Tissues Improves Cardiomyocyte Maturation
Henrique V. Almeida,Miguel F. Tenreiro,Ana F. Louro,Bernardo Abecasis,Deolinda Santinha,Tomás Calmeiro,Elvira Fortunato,Lino Ferreira,Paula M. Alves,Margarida Serra +9 more
- 29 Jan 2021
TL;DR: This study shows that human cardiac ECM functionalization of hiPSC-based cardiac tissues improves cardiomyocyte maturation, and provides essential insights to streamline the application of ECM in the development of hi PSC- based therapies targeting cardiac diseases.
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Online monitoring of hiPSC expansion and hepatic differentiation in 3D culture by dielectric spectroscopy.
Inês A. Isidro,Pedro C. Vicente,Daniel A. M. Pais,Joana Almeida,Mara Domingues,Bernardo Abecasis,Natalia Zapata-Linares,Juan R. Rodriguez-Madoz,Felipe Prosper,Anders Aspegren,Paula M. Alves,Margarida Serra +11 more
TL;DR: The results show a good correlation between the cell permittivity measured online and the aggregate biovolume measured by standard offline methods, demonstrating for the first time the potential of dielectric spectroscopy to monitor hiPSC expansion and differentiation in STBs.
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Toward a Microencapsulated 3D hiPSC-Derived in vitro Cardiac Microtissue for Recapitulation of Human Heart Microenvironment Features.
Bernardo Abecasis,Pedro G M Canhão,Henrique V. Almeida,Tomás Calmeiro,Elvira Fortunato,Patrícia Gomes-Alves,Margarida Serra,Paula M. Alves +7 more
TL;DR: This work explored a culture platform based on alginate microencapsulation and suspension culture systems to develop three-dimensional (3D) human cardiac microtissues, which entails the co-culture of human induced pluripotent stem cell (hiPSC) cardiac derivatives including aggregates of hiPSC–CM and single cells of hipsC–derived EC and MC (hi PSC–EC+MC).
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