In recent decades, the biomedical applications of mesenchymal stem cells (MSCs) have attracted increasing attention. MSCs are easily extracted from the bone marrow, fat, and synovium, and differentiate into various cell lineages according to the requirements of specific biomedical applications. As MSCs do not express significant histocompatibility complexes and immune stimulating molecules, they are not detected by immune surveillance and do not lead to graft rejection after transplantation. These properties make them competent biomedical candidates, especially in tissue engineering. We present a brief overview of MSC extraction methods and subsequent potential for differentiation, and a comprehensive overview of their preclinical and clinical applications in regenerative medicine, and discuss future challenges.

https://www.mdpi.com/2073-4409/8/8/886/pdf

Mesenchymal Stem Cells for Regenerative Medicine.

&NA; This study evaluates the feasibility of growing tissue‐engineered cartilage in the shape of a human ear using chondrocytes seeded onto a synthetic biodegradable polymer fashioned in the shape of a 3‐year‐old child's auricle. A polymer template was formed in the shape of a human auricle using a nonwoven mesh of polyglycolic acid molded after being immersed in a 1% solution of polylactic acid. Each polyglycolic acid‐polylactic acid template was seeded with chondrocytes isolated from bovine articular cartilage and then implanted into subcutaneous pockets on the dorsa of 10 athymic mice. The three‐dimensional structure was well maintained after removal of an external stent that had been applied for 4 weeks. Specimens harvested 12 weeks after implantation and subjected to gross morphologic and histologic analysis demonstrated new cartilage formation. The overall geometry of the experimental specimens closely resembled the complex structure of the child's auricle. These findings demonstrate that polyglycolic acid‐polylactic acid constructs can be fabricated in a very intricate configuration and seeded with chondrocytes to generate new cartilage that would be useful in plastic and reconstructive surgery. (Plast. Reconstr. Surg. 100: 297, 1997.)

Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear

Electrospinning for Tissue Engineering Applications

Nanotechnology refers to the fabrication, characterization, and application of substances in nanometer scale dimensions for various ends. The influence of nanotechnology on the healthcare industry is substantial, particularly in the areas of disease diagnosis and treatment. Recent investigations in nanotechnology for drug delivery and tissue engineering have delivered high-impact contributions in translational research, with associated pharmaceutical products and applications. Over the past decade, the synthesis of nanofibers or nanoparticles via electrostatic spinning or spraying, respectively, has emerged as an important nanostructuring methodology. This is due to both the versatility of the electrospinning/electrospraying process and the ensuing control of nanofiber/nanoparticle surface parameters. Electrosprayed nanoparticles and electrospun nanofibers are both employed as natural or synthetic carriers for the delivery of entrapped drugs, growth factors, health supplements, vitamins, and so on. The role of nanofiber/nanoparticle carriers is substantiated by the programmed, tailored, or targeted release of their contents in the guise of tissue engineering scaffolds or medical devices for drug delivery. This review focuses on the nanoformulation of natural materials via the electrospraying or electrospinning of nanoparticles or nanofibers for tissue engineering or drug delivery/pharmaceutical purposes. Here, we classify the natural materials with respect to their animal/plant origin and macrocyclic, small molecule or herbal active constituents, and further categorize the materials according to their proteinaceous or saccharide nature.

Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: applications in tissue regeneration, drug delivery and pharmaceuticals

Fatigue behaviour of FDM-3D printed polymers, polymeric composites and architected cellular materials

Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-e-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young’s modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.

/pdf/electrospun-gelatin-pcl-and-collagen-plcl-scaffolds-for-xdy3wn6gnp.pdf

Electrospun gelatin/PCL and collagen/PLCL scaffolds for vascular tissue engineering

Aim: To study the feasibility of electrospun collagen–poly(L-lactic acid-co-e-caprolactone) (collagen–PLCL) membranes for cartilage tissue engineering. Materials & methods: Characteristics and mechanical properties of collagen–PLCL membranes were analyzed. The cell affinity of collagen–PLCL membranes with chondrocytes was also assessed. Then, the cell–scaffold constructs were engineered with collagen–PLCL membranes seeded chondrocytes by a sandwich model. After culture for 1 week in vitro, the constructs were implanted subcutaneously into nude mice for 4, 8 and 12 weeks, followed by evaluation of the quality of neocartilage. Results: Collagen–PLCL membranes exhibited excellent balanced properties without cytotoxicity. With the extension of implantation time in vivo, the constructs revealed more cartilage-like tissue especially at 8 and 12 weeks. The Young’s modulus of the constructs also significantly increased and neared that of native cartilage at 12 weeks postimplantation. Conclusion: We suggest that c...

Electrospun collagen-poly(L-lactic acid-co-ε-caprolactone) membranes for cartilage tissue engineering.

Citric modified chitosan (CC) hydrogel containing antibacterial drugs is developed by the freezing and thawing treatment method. The SEM image of the CC hydrogel revealed a porous structure. The rheological properties, porosity, swelling rate, water uptake, tensile properties and in vitro degradation were found to be tunable via CC concentration. To enhance antibacterial properties, tetracycline hydrochloride (TH) representing the drug model, was integrated into the CC hydrogel. The cumulative release of drug was also tunable via CC concentration. The drug loaded CC hydrogel showed enhanced antimicrobial activity against E. coli and S. aureus. In animal tests, it was found the TH loaded CC hydrogel accelerated the healing of the wounds created on rats. These results suggest that the drug loaded CC hydrogel has a promising future in wound healing as a wound dressing.

/pdf/tetracycline-hydrochloride-loaded-citric-acid-functionalized-2j30k2g24g.pdf

Tetracycline hydrochloride loaded citric acid functionalized chitosan hydrogel for wound healing

Background
Myocardial infarction remains the leading cause of mortality in developed countries despite recent advances in its prevention and treatment. Regenerative therapies based on resident cardiac progenitor cells (CPCs) are a promising alternative to conventional treatments. However, CPCs resident in the heart are quite rare. It is unclear how these CPCs can be isolated and cultured efficiently and what the effects of long-term culture in vitro are on their ‘stemness’ and differentiation potential, but this is critical knowledge for CPCs’ clinical application.

/pdf/isolation-and-characterization-of-a-sca-1-cd31-progenitor-knfd647i47.pdf

Isolation and characterization of a Sca-1+/CD31-progenitor cell lineage derived from mouse heart tissue

The invention relates to a tracheal stent and application thereof. The tracheal stent comprises supporting rings and a supporting connecting rod, wherein the various supporting rings are of C-shaped structures and are serially connected by virtue of the supporting connecting rod, so that the various supporting rings are integrally fixed with the supporting connecting rod. The invention further provides a tissue engineering trachea, and the tissue engineering trachea is prepared by winding a chondrocyte-gelatin/polycaprolactone electrospun membrane complex on the outer surface of the tracheal stent. The tracheal stent or the tissue engineering trachea disclosed by the invention can be used for repairing tracheal stenosis by virtue of a tracheal external support suspension method, and a new C-shaped cartilaginous ring is formed after the implantation of the tracheal stent or the tissue engineering trachea, so as to guarantee growth potential finally.

Tracheal stent and application thereof

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