Ayako Ohbuchi
Himeji Dokkyo University
5 Papers
6 Citations
Ayako Ohbuchi is an academic researcher from Himeji Dokkyo University. The author has contributed to research in topics: Neutrophil extracellular traps & Chemistry. The author has an hindex of 3, co-authored 5 publications.
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Papers
Haemin-induced cell death in human monocytic cells is consistent with ferroptosis
Shion Imoto,Mari Kono,Takashi Suzuki,Yukiko Shibuya,Tohru Sawamura,Yuji Mizokoshi,Hirohide Sawada,Ayako Ohbuchi,Katsuyasu Saigo +8 more
TL;DR: The findings support haemin-induced cell death as an example of ferroptosis, and suggest ferroPTosis inhibitors may be useful for the treatment or prevention of transfusion iron overload.
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Quantitative analysis of hemin-induced neutrophil extracellular trap formation and effects of hydrogen peroxide on this phenomenon.
TL;DR: Quantitative analysis of NET formation may prove to be a useful tool for investigating NET physiology, and hemin could function as a possible therapeutic target for hemolysis-related events.
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Acetate moderately attenuates the generation of neutrophil extracellular traps
TL;DR: The linearity was unaffected by the addition of 25 or 50 mM acetate, and no statistically significant difference was observed between the data with and without acetate addition (N=6).
After haemin treatment intracellular non-haem iron increases prior to haem oxygenase-1 induction: A study in human monocytic cell line THP-1.
Shion Imoto,Yukiko Shibuya,Mari Kono,Ayako Ohbuchi,Tohru Sawamura,Takashi Suzuki,Yuji Mizokoshi,Hirohide Sawada,Katsuyasu Saigo +8 more
TL;DR: Increase in non-haem iron prior to induction ofHO-1 expression suggests the involvement of HO-2 in haem-induced cytotoxicity, which is consistent with ferroptosis, an iron-dependent cell death regulation mechanism.
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Labile iron, ROS, and cell death are prominently induced by haemin, but not by non-transferrin-bound iron.
Shion Imoto,Tohru Sawamura,Yukiko Shibuya,Mari Kono,Ayako Ohbuchi,Takashi Suzuki,Yuji Mizokoshi,Katsuyasu Saigo +7 more
TL;DR: In this paper, the intracellular labile iron pool (LIP) was measured using an iron-sensitive fluorescent probe, and the difference in LIP suggests the different iron traffic mechanisms for haem-derived iron and NTBI.
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