Phylogenetic relationship and mode of evolution of yeast DNA topoisomerase II gene in the pathogenic Candida species.

Chun Li (2004) Genetic analysis of two subspecies of Reevesmuntjac (Cervidae: Muntiacus reevesi) by kary- otyping and satellite DNA analyses. Zoological Studies 43(4): 749-758. We analyzed the karyotypes of the Formosan muntjac (Muntiacus reevesi micrurus) including G-banding, C-banding, and NOR-staining analyses. The results showed the species has a 2n = 46 chromosome complement. The G-banding patterns as well as the localizations of rRNA gene clusters and constitutive heterochromatins were similar to those of Chinese muntjac (M. reevesi reevesi). In addition, satellite DNA analysis was also carried out. The restriction periodicity of FM-satI revealed a 0.75-kb register indicating that this deer species belongs to the plesiometacarpalia divi- sion. Finally, the FISH study demonstrated that the Formosan and Chinese muntjacs have similar localizations of satellite I DNA in their respective genomes. Although the Formosan and Chinese muntjacs share almost identical results of cytogenetic analyses, Southern blot and FISH studies revealed some sequence divergence of satellite I DNA between these 2 species supporting the classification of the Formosan muntjac as a sub- species of, not the same species as, the Chinese muntjac. Furthermore, the data suggest that satellite I DNA of the Formosan muntjac and that of the Chinese muntjac may have originated from different ancestral sequences or that they may have experienced different homogenization patterns in the course of evolution. http://www.sinica.edu.tw/zool/zoolstud/43.4/749.pdf

Genetic analysis of two subspecies of Reeves' muntjac (Cervidae: Muntiacus reevesi) by karyotyping and satellite DNA analyses

Nucleotide sequence of BamHI family satellite DNA and its unit length polymorphism in bluegill sunfish Lepomis macrochirus (Copies of English Papers by the 〔Water Resources Research〕Center Staff Published in the Fiscal Year of 2000)

Satellite DNA sequences are known to be highly variable and to have been subjected to concerted evolution that homogenizes member sequences within species. We have analyzed the mode of evolution of satellite DNA sequences in four fishes from the genusDiplodus by calculating the nucleotide frequency of the sequence array and the phylogenetic distances between member sequences. Calculation of nucleotide frequency and pairwise sequence comparison enabled us to characterize the divergence among member sequences in this satellite DNA family. The results suggest that the evolutionary rate of satellite DNA inD. bellottii is about two-fold greater than the average of the other three fishes, and that the sequence homogenization event occurred inD. puntazzo more recently than in the others. The procedures described here are effective to characterize mode of evolution of satellite DNA.

/pdf/evaluation-of-intra-and-interspecific-divergence-of-u5gu0yhvnn.pdf

Evaluation of intra- and interspecific divergence of satellite DNA sequences by nucleotide frequency calculation and pairwise sequence comparison.

We have isolated and sequenced a member of tandem repetitive DNA containing BamHI site (BamHI family satellite DNA) from bluegill sunfish Lepomis macrochirus. PCR amplification with specific primers was performed to define the size of unit length repeat of the BamHI family satellite DNA, revealing that there were two distinct size of DNA fragments (0.9 kb and 1.3 kb) in the PCR products. The longer fragment (1.3 kb) consisted of internal sub-duplication of shorter fragment (0.9 kb). We have compared the size of PCR products among four fish populations, and found that both fragments co-existed in one population whereas the longer fragment was dominant in other three populations. The results may reflect ongoing homogenization of satellite DNA type over a short evolutionary time scale.

Nucleotide sequence of BamHI family satellite DNA and its unit length polymorphism in bluegill sunfish Lepomis macrochirus.

A Method for Evaluating Phylogenetic Relationship of α-Satellite DNA Suprachromosomal Family by Nucleotide Frequency Calculation

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A Method for Evaluating Phylogenetic Relationship of α-Satellite DNA Suprachromosomal Family by Nucleotide Frequency Calculation