Tri-methylation of lysine 4 on histone H3 (H3K4me3) is a near-universal chromatin modification at the transcription start site of active genes in eukaryotes from yeast to man and its levels reflect the amount of transcription. Because of this association, H3K4me3 is often described as an 'activating' histone modification and assumed to have an instructive role in the transcription of genes, but the field is lacking a conserved mechanism to support this view. The overwhelming finding from genome-wide studies is that actually very little transcription changes upon removal of most H3K4me3 under steady-state or dynamically changing conditions, including at mammalian CpG island promoters. Instead, rather than a major role in instructing transcription, time-resolved experiments provide more evidence supporting the deposition of H3K4me3 into chromatin as a result of transcription, influencing processes such as memory of previous states, transcriptional consistency between cells in a population and transcription termination.

/pdf/is-h3k4me3-instructive-for-transcription-activation-tw6bnu81j8.pdf

Is H3K4me3 instructive for transcription activation

What is Phenotypic Plasticity and Why is it Important? Phenotypic Plasticity and the Semantics of Polythenism: A Historical Review and Current Perspectives Phenotypic Plasticity and the Origins of Diversity: A Case Study on Horned Beetles Developmental Flexibility, Phenotypic Plasticity, and Host Plants: A Case Study with Nemoria Caterpillars Phase Polyphenism in Locusts: Mechanisms, Population, Consequences, Adaptive Significance and Evolution Density-Dependent Prophylaxis in Insects Natural Enemy-Induced Plasticity in Plants and Animals A Behaviorally Plasticity Response to Risk of Predation: Oviposition Site Selection by a Mosquito in Response to its Predators Polyphenisms in Lepidoptera: Multidisciplinary Approaches to Studies of Evolution and Development Causes and Consequences of Phenotypic Plasticity in Body Size: The Case of the Yellow Dung Fly Scathophaga stercoraria (Diptera: Scathophagidae She Shapes Events as they Come: Plasticity in Female Insect Reproduction Temperature Dependence of Development Rate, Growth Rate and Size: From Biophysics to Adaptation The Developmental-Physiological Basis of Phenotypic Plasticity Wing Polymorphism in Gryllus (Orthoptera: Gryllidae) Proximate Endocrine, Energetic and Biochemical Mechanisms Underlying Morph Specialization for Flight vs. Reproduction Evolution of Homeostatic Physiological Systems Acclimation as Adaptive Phenotypic Plasticity Heat Shock Proteins and their Role in Generating, Maintaining and Even Preventing Alternative Insect Phenotypes Learned Host Preferences Adaptive Maternal Effects: A Case Study of Egg Size Plasticity in a Seed-Feeding Bettle On the Origins of Insect Hormone Signaling Phenotypic Plasticity and Evolvability: An Empirical Test with Experimental Evolution

/pdf/phenotypic-plasticity-of-insects-mechanisms-and-consequences-18x7tgqort.pdf

Phenotypic plasticity of insects: mechanisms and consequences.

Large-Scale Genetic Perturbations Reveal Regulatory Networks and an Abundance of Gene-Specific Repressors

Spp1, a Member of the Set1 Complex, Promotes Meiotic DSB Formation in Promoters by Tethering Histone H3K4 Methylation Sites to Chromosome Axes

A central aspect of aging research concerns the question of when individuality in lifespan arises1. Here we show that a transient increase in reactive oxygen species (ROS), which occurs naturally during early development in a subpopulation of synchronized Caenorhabditis elegans, sets processes in motion that increase stress resistance, improve redox homeostasis and ultimately prolong lifespan in those animals. We find that these effects are linked to the global ROS-mediated decrease in developmental histone H3K4me3 levels. Studies in HeLa cells confirmed that global H3K4me3 levels are ROS-sensitive and that depletion of H3K4me3 levels increases stress resistance in mammalian cell cultures. In vitro studies identified SET1/MLL histone methyltransferases as redox sensitive units of the H3K4-trimethylating complex of proteins (COMPASS). Our findings implicate a link between early-life events, ROS-sensitive epigenetic marks, stress resistance and lifespan.

/pdf/developmental-ros-individualizes-organismal-stress-3mvv1gwhy5.pdf

Developmental ROS individualizes organismal stress resistance and lifespan

Packaging of eukaryotic genomes into chromatin has wide-ranging effects on gene transcription. Curiously, it is commonly observed that deletion of a global chromatin regulator affects expression of only a limited subset of genes bound to or modified by the regulator in question. However, in many single-gene studies it has become clear that chromatin regulators often do not affect steady-state transcription, but instead are required for normal transcriptional reprogramming by environmental cues. We therefore have systematically investigated the effects of 83 histone mutants, and 119 gene deletion mutants, on induction/repression dynamics of 170 transcripts in response to diamide stress in yeast. Importantly, we find that chromatin regulators play far more pronounced roles during gene induction/repression than they do in steady-state expression. Furthermore, by jointly analyzing the substrates (histone mutants) and enzymes (chromatin modifier deletions) we identify specific interactions between histone modifications and their regulators. Combining these functional results with genome-wide mapping of several histone marks in the same time course, we systematically investigated the correspondence between histone modification occurrence and function. We followed up on one pathway, finding that Set1-dependent H3K4 methylation primarily acts as a gene repressor during multiple stresses, specifically at genes involved in ribosome biosynthesis. Set1-dependent repression of ribosomal genes occurs via distinct pathways for ribosomal protein genes and ribosomal biogenesis genes, which can be separated based on genetic requirements for repression and based on chromatin changes during gene repression. Together, our dynamic studies provide a rich resource for investigating chromatin regulation, and identify a significant role for the “activating” mark H3K4me3 in gene repression.

Systematic Dissection of Roles for Chromatin Regulators in a Yeast Stress Response

/pdf/systematic-dissection-of-roles-for-chromatin-regulators-in-a-1a6n2d6czi.pdf

Systematic dissection of roles for chromatin regulators in a yeast stress response.

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Avital Klien

Author Tools

Chat about Author

Papers

Systematic Dissection of Roles for Chromatin Regulators in a Yeast Stress Response

Systematic dissection of roles for chromatin regulators in a yeast stress response.