Ashley M. Floyd
Wayne State University
5 Papers
18 Citations
Ashley M. Floyd is an academic researcher from Wayne State University. The author has contributed to research in topics: DNA repair & Cisplatin. The author has an hindex of 4, co-authored 5 publications.
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Papers
Targeting the DNA Repair Endonuclease ERCC1-XPF with Green Tea Polyphenol Epigallocatechin-3-Gallate (EGCG) and Its Prodrug to Enhance Cisplatin Efficacy in Human Cancer Cells
Joshua Heyza,Sanjeevani Arora,Hao Zhang,Kayla L. Conner,Wen Lei,Ashley M. Floyd,Rahul Deshmukh,Jeffrey G. Sarver,Christopher J. Trabbic,Paul W. Erhardt,Tak Hang Chan,Tak Hang Chan,Q. Ping Dou,Steve M. Patrick +13 more
TL;DR: This study identified the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) as a potent inhibitor of ERCC1/XPF activity with an IC50 (half maximal inhibitory concentration) in the nanomolar range in biochemical assays and shows that EGCG can inhibit DNA repair and enhance cisplatin sensitivity in human cancer cells.
Differential role of base excision repair proteins in mediating cisplatin cytotoxicity
TL;DR: The results indicate that BER activation at cisplatin ICLs influences crosslink repair and modulates cisPlatin cytotoxicity via specific UNG, APE1 and Polβ polymerase functions.
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A tRNA-independent Mechanism for Transamidosome Assembly Promotes Aminoacyl-tRNA Transamidation
Gayathri N. Silva,Shirin Fatma,Ashley M. Floyd,Frédéric Fischer,Pitak Chuawong,Pitak Chuawong,Amanda N. Cruz,Rachel M. Simari,Nilesh Joshi,Daniel Kern,Tamara L. Hendrickson +10 more
TL;DR: A novel protein partner called Hp0100 is identified as a component of a stable, tRNA-independent H. pylori Asn-transamidosome; this complex contains a non-discriminating aspartyl-tRNA synthetase, AdT, and Hp0200 but does not require tRNAAsn for assembly.
20
APOBEC3 enzymes mediate efficacy of cisplatin and are epistatic with base excision repair and mismatch repair in platinum response
Kayla L. Conner,Asra N. Shaik,Katie A Marshall,Ashley M. Floyd,Elmira Ekinci,Jacob Lindquist,Akshada Sawant,Wen Lei,Madison B. Adolph,Linda Chelico,Sachini U. Siriwardena,Ashok S. Bhagwat,Seongho Kim,Michele L. Cote,Steve M. Patrick +14 more
- 06 Nov 2020
TL;DR: APOBEC3 (A3) enzymes are shown to be capable of deaminating the extrahelical cytosines to uracils and sensitizing breast cancer cells to cisplatin, and it is proposed that A3-induced cytosine deamination to urACil at cis Platin ICLs results in repair of uracil by BER, which blocks ICL DNA repair and enhances cis platin efficacy and improves breast cancer outcomes.
Identification of small molecule inhibitors of ERCC1-XPF that inhibit DNA repair and potentiate cisplatin efficacy in cancer cells
Sanjeevani Arora,Joshua Heyza,Hao Zhang,Vivian Kalman-Maltese,Kristin Tillison,Ashley M. Floyd,Elaine M. Chalfin,Gerold Bepler,Steve M. Patrick +8 more
TL;DR: A high throughput screen used to identify small molecules that inhibit the endonuclease activity of ERCC1-XPF and found related compounds for one of the original Hits, which potentiated cisplatin cytotoxicity in cancer cells.