Ashley E. Sproles
Victoria University of Wellington
6 Papers
Ashley E. Sproles is an academic researcher from Victoria University of Wellington. The author has contributed to research in topics: Symbiodinium & Gene. The author has an hindex of 4, co-authored 4 publications.
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Papers
Menthol-induced bleaching rapidly and effectively provides experimental aposymbiotic sea anemones (Aiptasia sp.) for symbiosis investigations.
Jennifer L. Matthews,Ashley E. Sproles,Clinton A. Oakley,Arthur R. Grossman,Virginia M. Weis,Simon K. Davy +5 more
TL;DR: The ability of Aiptasia to form a stable symbiosis appeared to be unaffected by menthol exposure, as demonstrated by successful re-establishment of the symbiosis when anemones were experimentally re-infected.
Proteomics quantifies protein expression changes in a model cnidarian colonised by a thermally tolerant but suboptimal symbiont
Ashley E. Sproles,Clinton A. Oakley,Jennifer L. Matthews,Lifeng Peng,Jeremy G. Owen,Arthur R. Grossman,Virginia M. Weis,Simon K. Davy +7 more
TL;DR: The results provide targets for specific experiments to elucidate the mechanisms underpinning compensatory physiology in the coral–dinoflagellate symbiosis and predict pathways that may be involved in nutrient transport or resource allocation between partners.
Phylogenetic characterization of transporter proteins in the cnidarian-dinoflagellate symbiosis
Ashley E. Sproles,Nathan L. Kirk,Sheila A. Kitchen,Clinton A. Oakley,Arthur R. Grossman,Virginia M. Weis,Simon K. Davy +6 more
TL;DR: This study is the first to identify transporter sequences from a diversity of cnidarian species and Symbiodinium clades, which will be useful for future experimental analyses of the host-symbiont proteome and the nutritional exchange of Symbiod inium cells in hospite.
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Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins
TL;DR: In this paper , an optimized vector for the expression of recombinant proteins in Chlamydomonas reinhardtii was designed, which when transformed and screened using a dual antibiotic selection, followed by screening using fluorescence activated cell sorting (FACS), permits rapid identification and isolation of microalgal transformants with high expression of a recombinant protein.
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Novel cis-regulatory elements as synthetic promoters to drive recombinant protein expression from the Chlamydomonas reinhardtii nuclear genome.
TL;DR: In this article , 13 putative motifs were synthesized as concatemers, linked to a common minimal basal promoter, and assayed for their activity to drive expression of a yellow fluorescent protein reporter gene.
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