Asako Murayama
National Institutes of Health
53 Papers
579 Citations
Asako Murayama is an academic researcher from National Institutes of Health. The author has contributed to research in topics: Virus & Hepatitis C virus. The author has an hindex of 17, co-authored 46 publications. Previous affiliations of Asako Murayama include Toray Industries.
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Papers
Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
Takahiro Masaki,Ryosuke Suzuki,Kyoko Murakami,Hideki Aizaki,Koji Ishii,Asako Murayama,Tomoko Date,Yoshiharu Matsuura,Tatsuo Miyamura,Takaji Wakita,Tetsuro Suzuki +10 more
TL;DR: It is demonstrated that NS5A is a prerequisite for HCV particle production as a result of its interaction with the viral capsid protein (core protein), and that the alanine substitutions in the serine cluster suppressed the association of the core protein with viral genome RNA, possibly resulting in the inhibition of nucleocapsid assembly.
411
Cell culture and infection system for hepatitis C virus
TL;DR: This protocol describes how to use the JFH-1 viral culture system, which provides a powerful tool for studying viral life cycle and for the construction of antiviral strategies and the development of effective vaccines.
196
CD81 Expression Is Important for the Permissiveness of Huh7 Cell Clones for Heterogeneous Hepatitis C Virus Infection
Daisuke Akazawa,Tomoko Date,Kenichi Morikawa,Asako Murayama,Michiko Miyamoto,Minako Kaga,Heidi Barth,Thomas Baumert,Jean Dubuisson,Takaji Wakita +9 more
TL;DR: CD81 expression is an important determinant of HCV permissiveness of Huh7 cell clones harboring different characteristics, and was down-regulated upon transfection of CD81 silencing RNA into a CD81-positive cell clone.
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The Roles of CD81 and Glycosaminoglycans in the Adsorption and Uptake of Infectious HCV Particles
Kenichi Morikawa,Zijiang Zhao,Tomoko Date,Michiko Miyamoto,Asako Murayama,Daisuke Akazawa,Jun-Ichi Tanabe,Saburo Sone,Takaji Wakita +8 more
TL;DR: HSPG may play a role in the initial cell surface binding of infectious HCV particles and CD81 is conceivably correlated with HCV entry after viral attachment, suggesting that both HSPG andCD81 are important forHCV entry.
83
Involvement of Hepatitis C Virus NS5A Hyperphosphorylation Mediated by Casein Kinase I-α in Infectious Virus Production
Takahiro Masaki,Satoko Matsunaga,Hirotaka Takahashi,Kenji Nakashima,Yayoi Kimura,Masahiko Ito,Mami Matsuda,Asako Murayama,Takanobu Kato,Hisashi Hirano,Yaeta Endo,Stanley M. Lemon,Takaji Wakita,Tatsuya Sawasaki,Tetsuro Suzuki +14 more
TL;DR: In this paper, the authors identified the region within the low-complexity sequence I of Nonstructural Protein 5A (NS5A) that is involved in NS5A hyperphosphorylation and its exact function in the hepatitis C virus (HCV) life cycle.
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