http://www.cell.com/article/S1934590922004519/pdf

A reference human induced pluripotent stem cell line for large-scale collaborative studies.

Characterization of the tumor microenvironment through immunoprofiling has become an essential resource for the understanding of the complex immune cell interactions and the assessment of biomarkers for prognosis and prediction of immunotherapy response; however, these studies are often limited by tissue heterogeneity and sample size. The nanoString GeoMx® Digital Spatial Profiler (DSP) is a platform that allows high-plex profiling at the protein and RNA level, providing spatial and temporal assessment of tumors in frozen or formalin-fixed paraffin-embedded limited tissue sample. Recently, high-impact studies have shown the feasibility of using this technology to identify biomarkers in different settings, including predictive biomarkers for immunotherapy in different tumor types. These studies showed that compared to other multiplex and high-plex platforms, the DSP can interrogate a higher number of biomarkers with higher throughput; however, it does not provide single-cell resolution, including co-expression of biomarker or spatial information at the single-cell level. In this review, we will describe the technical overview of the platform, present current evidence of the advantages and limitations of the applications of this technology, and provide important considerations for the experimental design for translational immune-oncology research using this tissue-based high-plex profiling approach. 

/pdf/challenges-and-opportunities-for-immunoprofiling-using-a-h3ie6ymn.pdf

Challenges and Opportunities for Immunoprofiling Using a Spatial High-Plex Technology: The NanoString GeoMx® Digital Spatial Profiler

Tissue wounding causes rapid mechanical changes in the epithelium, but how these changes affect intracellular organization remains unclear. Using the Drosophila embryonic epidermis, we show that wounding induces a fast, transient compaction of chromatin and its detachment (dewetting) from the nuclear lamina in about half of the wound-edge cells. Within minutes, chromatin in these cells re-expands and re-associates with the lamina (rewetting). This reversible chromatin lamina dewetting is mechanosensitive, requiring both the LINC complex and intracellular calcium. During compaction, calcium is stored within chromatin and is later released during ATR mediated expansion and lamina re-wetting. Chromatin re-expansion then triggers actomyosin contraction and promotes tissue repair through Jun kinase activation, straightening of the wound edge, and supracellular actin ring formation. Together, our findings reveal a multiscale mechanosensitive mechanism by which tissue-scale mechanical changes induce a reversible chromatin-lamina dewetting, leading to cellular contraction and initiation of tissue repair. 

Mechanosensitive and Reversible Chromatin-Lamina Dewetting Triggers Cellular Contraction During Wound Healing

Mechanomedicine in anastomosis: Bridging biomechanics and clinical outcomes for improved surgical healing

Causal effects of skin microbiota on hypertrophic scars via cytokines and immune phenotypes

Inhibiting mechanotransduction prevents scarring and yields regeneration in a large animal model.

Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology

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Inhibiting mechanotransduction prevents scarring and yields regeneration in a large animal model.

Multiplex imaging of human induced pluripotent stem cell-derived neurons with CO-Detection by indEXing (CODEX) technology