Anna Joëlle Ruff
RWTH Aachen University
49 Papers
137 Citations
Anna Joëlle Ruff is an academic researcher from RWTH Aachen University. The author has contributed to research in topics: Chemistry & Saturated mutagenesis. The author has an hindex of 13, co-authored 42 publications. Previous affiliations of Anna Joëlle Ruff include Forschungszentrum Jülich & Graz University of Technology.
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Papers
Lessons from diversity of directed evolution experiments by an analysis of 3,000 mutations
TL;DR: The diversity in random mutagenesis libraries employed in directed evolution experiments was determined and the chemical composition of the amino acid substitutions differed, however, significantly from the two epPCR methods to SeSaM‐Tv P/P.
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Flow Cytometer-Based High-Throughput Screening System for Accelerated Directed Evolution of P450 Monooxygenases
TL;DR: The first whole-cell, high-throughput screening platform for P450 monooxygenases based on flow cytometry is reported, which can likely be applied to directed evolution campaigns of any P450 Monooxygenase that catalyzes the O-dealkylation of BCCE.
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Directed Evolution of P 450 BM 3 into a p‐Xylene Hydroxylase
TL;DR: The semi-rational engineering of the self-sufficient monooxygenase P 450 BM 3 towards the direct aromatic hydroxylation of p-xylene is described, which is of high interest for the production of temperature stable polymers (>500 K), as is 2,5-DMP itself, which can act as a building block for “next-generation” plastics.
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An Enzymatic Route to α‐Tocopherol Synthons: Aromatic Hydroxylation of Pseudocumene and Mesitylene with P450 BM3
Alexander Dennig,Alexandra Maria Weingartner,Tsvetan Kardashliev,Christina Andrea Müller,Erika Tassano,Martin Schürmann,Anna Joëlle Ruff,Ulrich Schwaneberg,Ulrich Schwaneberg +8 more
TL;DR: This study provides an enzymatic route to key phenolic synthons for α-tocopherols and the first catalytic and mechanistic insights into direct aromatic hydroxylation and dearomatization of trimethylbenzenes with O2.
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Engineered phytases for emerging biotechnological applications beyond animal feeding
TL;DR: The main challenge in designing phytases to completely hydrolyze phosphate from phytate to inositol and the need for engineering campaigns to broaden their industrial use are described.
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