Andreja Jovic

TL;DR: In this article, phase-locking analysis of the M3 muscarinic receptor-calcium signaling pathway was used to reveal the potential importance of basal IP3 production, a finding that has important implications on calcium response fidelity to periodic stimulation.

TL;DR: Improvements can be made to fluidic systems that provide the means to analyze and manipulate cellular behavior in dynamic stimulatory environments on the single-cell level and on a high-throughput level to enhance their utility for high-impact biological investigations of temporal dynamics.

TL;DR: A combined theoretical and experimental analysis is presented that shows how to appropriately balance stimulation strength, duration, and rest intervals to achieve entrainment with high fidelity stimulation-to-response ratios for G-protein-coupled receptor-triggered intracellular calcium oscillations and suggests that, at key threshold values, even small changes in these protein concentrations or activities can result in precipitous changes in entrainments fidelity.

TL;DR: The resulting model suggests that dephosphorylation of deactivated receptors is rate limiting for recovery of calcium signals in the acute regime (high ligand concentration), while calcium replenishment and IP3 production determine signal recovery in the oscillatory regime (low ligand concentrations).