Amit Pathak
Washington University in St. Louis
48 Papers
233 Citations
Amit Pathak is an academic researcher from Washington University in St. Louis. The author has contributed to research in topics: Extracellular matrix & Medicine. The author has an hindex of 14, co-authored 30 publications. Previous affiliations of Amit Pathak include University of California, Santa Barbara & University of Pennsylvania.
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Papers
Independent regulation of tumor cell migration by matrix stiffness and confinement
Amit Pathak,Sanjay Kumar +1 more
TL;DR: A matrix platform based on microfabrication of channels of defined wall stiffness and geometry that allows independent variation of ECM stiffness and channel width is introduced and it is demonstrated that matrix confinement alters the relationship between cell migration speed andECM stiffness.
566
Microfabricated tissue gauges to measure and manipulate forces from 3D microtissues
Wesley R. Legant,Amit Pathak,Michael T. Yang,Vikram Deshpande,Robert M. McMeeking,Christopher S. Chen +5 more
TL;DR: It is demonstrated how intratissue gradients of mechanical stress can emerge from collective cellular contractility and finally, how such gradients can be used to engineer protein composition and organization within a 3D tissue.
Past matrix stiffness primes epithelial cells and regulates their future collective migration through a mechanical memory.
Samila Nasrollahi,Christopher Walter,Andrew J. Loza,Gregory V. Schimizzi,Gregory D. Longmore,Amit Pathak +5 more
TL;DR: It is reported that epithelial cells primed on a stiff matrix migrate faster, display higher actomyosin expression, form larger focal adhesions, and retain nuclear YAP even after arriving onto a soft secondary matrix, as compared to their control behavior on a homogeneously soft matrix.
159
The simulation of stress fibre and focal adhesion development in cells on patterned substrates.
TL;DR: The remodelling of the cytoskeleton and focal adhesion distributions for cells on substrates with micro-patterned ligand patches is investigated using a bio-chemo-mechanical model and accurately predicts the radii of curvature of the non-adhered edges of cells on the concave-shaped ligand patterns.
130
DDR2 controls breast tumor stiffness and metastasis by regulating integrin mediated mechanotransduction in CAFs.
Samantha Van Hove Bayer,Whitney R. Grither,Audrey Brenot,Priscilla Y. Hwang,Craig E. Barcus,Melanie Ernst,Patrick Pence,Christopher Walter,Amit Pathak,Gregory D. Longmore +9 more
TL;DR: It is shown in mouse breast tumors that the action of the collagen receptor DDR2 in CAFs controls tumor stiffness by reorganizing collagen fibers specifically at the tumor-stromal boundary.