Alexandra Thurston
University of Nottingham
6 Papers
17 Citations
Alexandra Thurston is an academic researcher from University of Nottingham. The author has contributed to research in topics: DNA methylation & Epigenetics. The author has an hindex of 6, co-authored 6 publications.
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Papers
Epigenetics and the germline
TL;DR: The consequences of epigenetic perturbation are discussed and new in vitro models which may allow further understanding of a difficult developmental period to study, especially in the human, are highlighted.
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Monoallelic expression of nine imprinted genes in the sheep embryo occurs after the blastocyst stage
TL;DR: Overall, the imprinting of ovine GRB10 and IGF2R was comparable with mouse but not with human, and contrary to the trophoblast-restricted maternal expression in both mouse and human, SASH2 (sheep homologue of Mash2/HASH2) was expressed in the ovine foetus and was biallelically expression in the chorioallantois.
Region-specific DNA methylation in the preimplantation embryo as a target for genomic plasticity.
TL;DR: The distribution of cytosine methylation in the genome is explored and the potential effects of regional plasticity on subsequent development is discussed, enhancing understanding of how individuals are shaped not only by DNA sequence, but by the environment in which pluripotent embryonic cells are transformed into the many cell types of the body.
18
Gene-specific vulnerability to imprinting variability in human embryonic stem cell lines
Kee-Pyo Kim,Alexandra Thurston,Christine L. Mummery,Dorien Ward-van Oostwaard,Helen Priddle,Cinzia Allegrucci,Chris Denning,Lorraine E. Young +7 more
TL;DR: Gene-specific differences in the stability of imprinted loci in human embryonic stem cells are demonstrated and disrupted DNA methylation is identified as one potential mechanism for disrupted imprinting.
Restriction landmark genome scanning identifies culture-induced DNA methylation instability in the human embryonic stem cell epigenome
Cinzia Allegrucci,Yue Zhong Wu,Alexandra Thurston,Chris Denning,Helen Priddle,Christine L. Mummery,Dorien Ward-van Oostwaard,Peter W. Andrews,Miodrag Stojkovic,Nigel Smith,Tony Parkin,Mark Edmondson Jones,Graham Warren,Li Yu,Romulo M. Brena,Christoph Plass,Lorraine E. Young +16 more
TL;DR: Current methods of hESC propagation can rapidly programme stable and unpredictable epigenetic changes in the stem cell genome, which highlights the need for novel screening strategies and standardization of procedures for the derivation and culture of h ESC lines that minimize culture-induced instability.